Analytical Data
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Gene name
PKR1
- Application
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Alternative Names
PKR1；GPR73；PKR1；Prokineticin receptor 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TCW9
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Expression Region
1-393aa
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AA Sequence
METTMGFMDDNATNTSTSFLSVLNPHGAHATSFPFNFSYSDYDMPLDEDEDVTNSRTFFAAKIVIGMALVGIMLVCGIGNFIFIAALVRYKKLRNLTNLLIANLAISDFLVAIVCCPFEMDYYVVRQLSWEHGHVLCTSVNYLRTVSLYVSTNALLAIAIDRYLAIVHPLRPRMKCQTATGLIALVWTVSILIAIPSAYFTTETVLVIVKSQEKIFCGQIWPVDQQLYYKSYFLFIFGIEFVGPVVTMTLCYARISRELWFKAVPGFQTEQIRKRLRCRRKTVLVLMCILTAYVLCWAPFYGFTIVRDFFPTVFVKEKHYLTAFYIVECIAMSNSMINTLCFVTVKNDTVKYFKKIMLLHWKASYNGGKSSADLDLKTIGMPATEEVDCIRLK
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Molecular Weight
44.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PKR1, or Protein Kinase R (PKR), is a serine/threonine kinase that plays a crucial role in the antiviral response and regulation of cellular processes. It is activated in response to double-stranded RNA, a molecular pattern associated with viral infections, leading to the phosphorylation of eIF2α and subsequent inhibition of protein synthesis, which helps inhibit viral replication. The study of PKR1 has gained significance due to its involvement in various cellular signaling pathways, including apoptosis, inflammation, and cell growth. Dysregulation of PKR1 has been implicated in several diseases, including cancer and autoimmune disorders, highlighting its potential as a therapeutic target. The recombinant expression of PKR1 in vitro allows researchers to investigate its biochemical properties, interaction partners, and regulatory mechanisms. Furthermore, understanding its structure-function relationship can pave the way for the design of novel inhibitors or activators, contributing to the development of new therapeutic strategies against viral infections and related diseases. As such, the research on PKR1 recombinant proteins plays a vital role in elucidating the molecular underpinnings of various pathophysiological conditions and holds promise for advancing targeted therapeutic approaches.
