Analytical Data
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基因名
NOP58
- Application
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别名
NOP58；NOL5；NOP5；Nucleolar Protein 58
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y2X3
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表达区间
1-529aa
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氨基酸序列
MLVLFETSVGYAIFKVLNEKKLQEVDSLWKEFETPEKANKIVKLKHFEKFQDTAEALAAFTALMEGKINKQLKKVLKKIVKEAHEPLAVADAKLGGVIKEKLNLSCIHSPVVNELMRGIRSQMDGLIPGVEPREMAAMCLGLAHSLSRYRLKFSADKVDTMIVQAISLLDDLDKELNNYIMRCREWYGWHFPELGKIISDNLTYCKCLQKVGDRKNYASAKLSELLPEEVEAEVKAAAEISMGTEVSEEDICNILHLCTQVIEISEYRTQLYEYLQNRMMAIAPNVTVMVGELVGARLIAHAGSLLNLAKHAASTVQILGAEKALFRALKSRRDTPKYGLIYHASLVGQTSPKHKGKISRMLAAKTVLAIRYDAFGEDSSSAMGVENRAKLEARLRTLEDRGIRKISGTGKALAKTEKYEHKSEVKTYDPSGDSTLPTCSKKRKIEQVDKEDEITEKKAKKAKIKVKVEEEEEEKVAEEEETSVKKKKKRGKKKHIKEEPLSEEEPCTSTAIASPEKKKKKKKKRENED
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分子量
75.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NOP58 is a crucial component of the nucleolar complex involved in ribosome biogenesis and RNA processing. As part of the box C/D small nucleolar ribonucleoproteins (snoRNPs), NOP58 plays a significant role in the methylation of ribosomal RNA (rRNA) and other RNA species, which is essential for the proper assembly of ribosomes. Its dysfunction has been linked to various diseases, including cancer and genetic disorders, making it a critical target for research. Recent studies have focused on the detailed characterization of NOP58's structure and function, utilizing recombinant protein technologies to produce and purify NOP58 for biochemical assays and structural biology studies. This research aims to shed light on the molecular mechanisms by which NOP58 and its associated snoRNPs regulate gene expression and ribosome assembly, as well as to explore potential therapeutic strategies for diseases associated with its dysfunction. Understanding the interactions and dynamics of NOP58 within the nucleolus not only enhances our knowledge of fundamental cellular processes but also opens avenues for innovative approaches in disease treatment and biotechnology.
