Analytical Data
-
Gene name
ERCC3
- Application
-
Alternative Names
(TFIIH subunit XPB)(Basic transcription factor 2 89 kDa subunit)(BTF2 p89)(DNA excision repair protein ERCC-3)(DNA repair protein complementing XP-B cells)(TFIIH basal transcription factor complex 89 kDa subunit)(TFIIH 89 kDa subunit)(TFIIH p89)(Xeroderma pigmentosum group B-complementing protein)
-
Species
Human
-
Source
E. coli
-
Tag
N- GST
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P19447
-
Expression Region
1-782aa
-
Molecular Weight
115.6 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ERCC3, also known as XPB, is a pivotal protein that plays a crucial role in the nucleotide excision repair (NER) pathway, which is essential for the cellular response to DNA damage caused by environmental factors such as UV radiation and chemical agents. Deficiencies in ERCC3 are linked to xeroderma pigmentosum (XP), a genetic disorder characterized by extreme sensitivity to sunlight and a predisposition to skin cancer. Research on ERCC3 recombinant protein focuses on understanding its structure, function, and interactions within the NER complex. By producing ERCC3 in vitro, scientists aim to elucidate its mechanism in DNA repair, explore its role in the cellular response to oxidative stress, and investigate how mutations in ERCC3 contribute to disorders like XP and other cancer-related conditions. Additionally, the study of recombinant ERCC3 provides insights into potential therapeutic strategies that leverage the protein's protective functions, aiding in the development of treatments for diseases linked to DNA repair deficiencies. This research not only enhances our understanding of NER but also opens avenues for innovative approaches to combatting the effects of DNA damage in various clinical settings.
