Cat: PA1000-9196

Recombinant Human CPO Protein,His

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Analytical Data

  • Gene name

    CPO

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    CPO;Carboxypeptidase O

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P36551

  • Expression Region

    111-454aa

  • AA Sequence

    TSLGRPEEEE DELAHRCSSF MAPPVTDLGE LRRRPGDMKT KMELLILETQ AQVCQALAQV DGGANFSVDR WERKEGGGGI SCVLQDGCVF EKAGVSISVV HGNLSEEAAK QMRSRGKVLK TKDGKLPFCA MGVSSVIHPK NPHAPTIHFN YRYFEVEEAD GNKQWWFGGG CDLTPTYLNQ EDAVHFHRTL KEACDQHGPD LYPKFKKWCD DYFFIAHRGE RRGIGGIFFD DLDSPSKEEV FRFVQSCARA VVPSYIPLVK KHCDDSFTPQ EKLWQQLRRG RYVEFNLLYD RGTKFGLFTP GSRIESILMS LPLTARWEYM HSPSENSKEA EILEVLRHPR DWVR

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

The study of CPO (cytochrome P450 oxidoreductase) recombinant proteins has garnered significant attention due to their indispensable role in various biological processes, particularly in drug metabolism and the biosynthesis of steroids and other biochemical molecules. CPO is a flavoprotein that serves as a crucial electron donor, facilitating the activity of cytochrome P450 enzymes, which are essential for the oxidative metabolism of a broad range of substrates, including pharmaceuticals, toxins, and endogenous compounds. Variations in CPO activity can lead to altered drug responses and toxicity, making it critical to understand its functionality. Advances in recombinant DNA technology have enabled the production of CPO proteins in heterologous systems, allowing for detailed studies on their biochemical properties, stability, and interactions with other metabolic enzymes. Investigating the structure-function relationship of CPO can provide insights into its mechanisms of action and potential applications in pharmacogenomics, toxicology, and biotechnology. With the increasing complexity of drug development and personalized medicine, understanding CPO's role opens avenues for the optimization of therapeutic regimens and the mitigation of adverse drug reactions, thus highlighting the significance of ongoing research in this domain.

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