Analytical Data
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Gene name
CBX5
- Application
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Alternative Names
CBX5；HP1A；Chromobox Protein homolog 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P45973
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Expression Region
1-191aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMGKKTK RTADSSSSED EEEYVVEKVL DRRVVKGQVE YLLKWKGFSE EHNTWEPEKN LDCPELISEF MKKYKKMKEG ENNKPREKSE SNKRKSNFSN SADDIKSKKK REQSNDIARG FERGLEPEKI IGATDSCGDL MFLMKWKDTD EADLVLAKEA NVKCPQIVIA FYEERLTWHA YPEDAENKEK ETAKS
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CBX5, also known as Chromobox protein homolog 5, is a member of the Polycomb Group (PcG) proteins, which play a crucial role in the regulation of gene expression and cellular identity through epigenetic mechanisms. CBX5 is specifically recognized for its function in maintaining transcriptional repression through histone methylation, particularly binding to trimethylated histone H3 at lysine 9 (H3K9me3). This protein is integral to the formation of heterochromatin, which helps stabilize gene silencing during development and cell differentiation. Research into CBX5 has garnered significant interest due to its involvement in various biological processes, including cell proliferation, differentiation, and the regulation of stem cell pluripotency. Abnormal expression or dysregulation of CBX5 has been associated with several diseases, including cancer, where it may contribute to tumor progression and metastasis by modulating key target genes responsible for cell cycle regulation and apoptosis. Consequently, the study of recombinant CBX5 protein has become essential in understanding its functional mechanisms and potential roles in therapeutic interventions. Researchers are exploring its binding affinities, structural properties, and interactions with other chromatin-associated proteins to elucidate its contribution to epigenetic regulation and to develop targeted strategies for cancer treatment and regenerative medicine. Overall, the investigation of CBX5 and its recombinant forms holds promise for advancing our understanding of epigenetic control mechanisms and their implications in health and disease.
