Analytical Data
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Gene name
TOP2
- Application
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Alternative Names
TOP2；TOP2；DNA topoisomerase 2-alpha
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P11388
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Expression Region
全长
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AA Sequence
full
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of topoisomerase II (TOP2) proteins has gained significant attention in the field of molecular biology and cancer research due to their essential roles in DNA topology maintenance during cellular processes such as replication, transcription, and chromosome segregation. TOP2 proteins exist as two isoforms, TOP2α and TOP2β, each exhibiting distinct functions and expression patterns across various tissues. TOP2α is predominantly expressed in proliferating tissues and is crucial for cell division, making it a key target in cancer therapies, especially for hematological malignancies and solid tumors. In contrast, TOP2β is more widely distributed and plays a role in transcription regulation and maintaining genomic stability. The inhibition of TOP2 activity, often achieved through the use of certain chemotherapeutic agents like anthracyclines, leads to the formation of DNA double-strand breaks, which ultimately triggers apoptosis in rapidly dividing cancer cells. However, the dual roles of TOP2 proteins in normal and cancerous cells highlight the importance of understanding their mechanisms thoroughly, as this knowledge can lead to the development of more targeted and effective therapies with fewer side effects. Consequently, ongoing research aims to elucidate the structural, functional, and regulatory aspects of TOP2 proteins, providing insights into their potential as therapeutic targets and advancing our comprehension of the complexities of cancer biology.
