Analytical Data
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基因名
BUB3
- Application
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别名
BUB3；Mitotic checkpoint Protein BUB3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43684
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表达区间
1-328aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMTGSNEF KLNQPPEDGI SSVKFSPNTS QFLLVSSWDT SVRLYDVPAN SMRLKYQHTG AVLDCAFYDP THAWSGGLDH QLKMHDLNTD QENLVGTHDA PIRCVEYCPE VNVMVTGSWD QTVKLWDPRT PCNAGTFSQP EKVYTLSVSG DRLIVGTAGR RVLVWDLRNM GYVQQRRESS LKYQTRCIRA FPNKQGYVLS SIEGRVAVEY LDPSPEVQKK KYAFKCHRLK ENNIEQIYPV NAISFHNIHN TFATGGSDGF VNIWDPFNKK RLCQFHRYPT SIASLAFSND GTTLAIASSY MYEMDDTEHP EDGIFIRQVT DAETKPKSPC T
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分子量
40 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Bub3, a critical component of the spindle assembly checkpoint (SAC), plays a vital role in maintaining genomic stability during cell division. As a member of the Bub protein family, Bub3 interacts with various proteins, including Bub1 and Mad3, to form complexes that inhibit the anaphase-promoting complex/cyclosome (APC/C) until all chromosomes are properly aligned at the metaphase plate. Misregulation of Bub3 and the SAC can lead to aneuploidy, a condition characterized by an abnormal number of chromosomes, which is frequently observed in cancer cells. Given the significant implications for tumorigenesis, understanding the molecular mechanisms governing Bub3's function and its interactions could provide insights into potential therapeutic targets for cancer treatment. Researchers have focused on studying the structural and functional aspects of Bub3, including its protein-protein interactions and regulatory pathways. Advances in techniques such as cryo-electron microscopy and mutagenesis have facilitated a deeper understanding of how Bub3 operates within the SAC. Furthermore, exploring the implications of Bub3 dysregulation in various cancer types has opened new avenues for targeted therapies, making it a critical focus in cancer biology and molecular genetics. Overall, the study of Bub3 not only enhances our understanding of cell division but also holds promise for the development of innovative cancer treatments.
