Analytical Data
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Gene name
GTF2E1
- Application
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Alternative Names
GTF2E1；TF2E1；General transcription factor IIE subunit 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P29083
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Expression Region
2-439aa
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AA Sequence
ADPDVLTEV PAALKRLAKY VIRGFYGIEH ALALDILIRN SCVKEEDMLE LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRHN YYFINYRTLV NVVKYKLDHM RRRIETDERD STNRASFKCP VCSSTFTDLE ANQLFDPMTG TFRCTFCHTE VEEDESAMPK KDARTLLARF NEQIEPIYAL LRETEDVNLA YEILEPEPTE IPALKQSKDH AATTAGAASL AGGHHREAWA TKGPSYEDLY TQNVVINMDD QEDLHRASLE GKSAKERPIW LRESTVQGAY GSEDMKEGGI DMDAFQEREE GHAGPDDNEE VMRALLIHEK KTSSAMAGSV GAAAPVTAAN GSDSESETSE SDDDSPPRPA AVAVHKREED EEEDDEFEEV ADDPIVMVAG RPFSYSEVSQ RPELVAQMTP EEKEAYIAMG QRMFEDLFE
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GTF2E1, a member of the general transcription factor IIE family, plays a critical role in the initiation phase of transcription by RNA polymerase II. It is involved in the formation of the transcription pre-initiation complex and influences the recruitment of RNA polymerase II and other essential factors. Research on GTF2E1 has gained momentum due to its significance in various biological processes, including cellular proliferation, differentiation, and response to stress. Additionally, abnormalities in GTF2E1 expression have been linked to several diseases, including cancer, where dysregulation of transcriptional activity can lead to uncontrolled cell growth. The study of recombinant GTF2E1 protein facilitates a deeper understanding of its biochemical properties, interaction with other transcription factors, and overall contribution to gene expression regulation. By generating and characterizing GTF2E1 in a recombinant form, researchers can investigate its functional mechanisms, paving the way for potential therapeutic interventions targeting transcriptional dysregulation in pathological conditions. Moreover, understanding its structure-function relationships can offer insights into developing small-molecule modulators to influence its activity, thereby presenting innovative strategies for cancer treatment and other diseases associated with transcriptional misregulation. Overall, the investigation of recombinant GTF2E1 not only enhances our comprehension of fundamental transcriptional processes but also holds promise for novel therapeutic applications in gene expression control.
