Analytical Data
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基因名
DOK7
- Application
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别名
DOK7；C4orf25；Protein Dok-7
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q18PE1
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表达区间
1-504aa
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氨基酸序列
MTEAALVEGQ VKLRDGKKWK SRWLVLRKPS PVADCLLMLV YKDKSERIKG LRERSSLTLE DICGLEPGLP YEGLVHTLAI VCLSQAIMLG FDSHEAMCAW DARIRYALGE VHRFHVTVAP GTKLESGPAT LHLCNDVLVL ARDIPPAVTG QWKLSDLRRY GAVPSGFIFE GGTRCGYWAG VFFLSSAEGE QISFLFDCIV RGISPTKGPF GLRPVLPDPS PPGPSTVEER VAQEALETLQ LEKRLSLLSH AGRPGSGGDD RSLSSSSSEA SHLDVSASSR LTAWPEQSSS SASTSQEGPR PAAAQAAGEA MVGASRPPPK PLRPRQLQEV GRQSSSDSGI ATGSHSSYSS SLSSYAGSSL DVWRATDELG SLLSLPAAGA PEPSLCTCLP GTVEYQVPTS LRAHYDTPRS LCLAPRDHSP PSQGSPGNSA ARDSGGQTSA GCPSGWLGTR RRGLVMEAPQ GSEATLPGPA PGEPWEAGGP HAGPPPAFFS ACPVCGGLKV NPPP
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DOK7, or Docking protein 7, is a critical component involved in neuromuscular junction (NMJ) development and synaptic signaling. It serves primarily as a scaffolding protein, facilitating the interaction between muscle-specific kinase (MuSK) and other signaling molecules that are essential for the formation and maintenance of NMJs. Mutations in the DOK7 gene have been linked to a form of congenital myasthenic syndrome, characterized by muscle weakness and fatigability, thereby underscoring its pivotal role in neuromuscular transmission. Research into DOK7 recombinant proteins has gained significant attention as these proteins provide valuable insights into the molecular mechanisms underlying NMJ formation and function. By utilizing recombinant DOK7, scientists aim to elucidate its interactions at the cellular level, explore its structural properties, and assess its role in signaling pathways that influence muscle contraction and synaptic stability. Furthermore, studying DOK7 in various model systems, including animal models and cell cultures, helps in understanding the disease mechanisms associated with DOK7 mutations and offers potential therapeutic avenues for neuromuscular disorders. Overall, the investigation of DOK7 recombinant proteins is vital for advancing our knowledge of NMJ biology and developing targeted interventions for conditions arising from its dysfunction.
