Analytical Data
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Gene name
IREB2
- Application
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Alternative Names
IREB2；Iron-responsive element-binding Protein 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48200
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Expression Region
1-343aa
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AA Sequence
MDAPKAGYAFEYLIETLNDSSHKKFFDVSKLGTKYDVLPYSIRVLLEAAV RNCDGFLMKKEDVMNILDWKTKQSNVEVPFFPARVLLQDFTGIPAMVDFA AMREAVKTLGGDPEKVHPACPTDLTVDHSLQIDFSKCAIQNAPNPGGGDL QKAGKLSPLKVQPKKLPCRGQTTCRGSCDSGELGRNSGTFSSQIENTPIL CPFHLQPVPEPETVLKNQEVEFGRNRERLQFFKWSSRVFKNVAVIPPGTG MAHQINLEYLSRVVFEEKDLLFPDSVVGTDSHITMVNGLGILGWGVGGIE TEAVMLGLPVSLTLPEVVGCELTGSSNPFVTSIDVVLGITKVS
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Molecular Weight
63 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
IREB2 (Iron Regulatory Element Binding Protein 2), a member of the IRP family, plays a pivotal role in cellular iron homeostasis by regulating the expression of genes involved in iron metabolism. It binds to iron-responsive elements (IREs) in the mRNA of various iron-related proteins, influencing their translation based on the cellular iron levels. Abnormal functioning of IREB2 can lead to disorders related to iron overload or deficiency, including anemia and neurodegenerative diseases. Recent research has emphasized its role not only in iron regulation but also in modulating cellular responses to oxidative stress and inflammation, suggesting a complex interplay between iron metabolism and various pathophysiological conditions. Understanding the biochemical mechanisms underlying IREB2 function can provide insights into potential therapeutic targets for diseases associated with iron dysregulation, thus making it a significant focus of contemporary biomedical research.
