Analytical Data
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Gene name
AST2
- Application
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Alternative Names
AST2；Protein AST2
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P39945
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Expression Region
1-430aa
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AA Sequence
MAEKILENKD PKLEAMTVDH EVSAPKPIPV DEPTLTRVAR PLRHVRHIPV KSLVFHSKHG PITFSYENKI KLPISKNKLV VQVNYVGLNP VDMKIRNGYT KPIYGEAGIG REYSGVITHV GDNLTNRWNV GDDVYGIYYH PKLAIGALQS SLLIDPRVDP ILMRPKNTLS PEKAAGSLFC LGTALNLLAQ LKEKDQLNTE SNVLINGGTS SVGMFAIQLL KRYYKVSKKL VVVTSGNGAA VLSEHFPDLK DEIIFINYLS CRGKSSKPLR RMLDTGKVVD YDDFNTLKET EDYTQGKFNV VLDFIGGYDI LSHSSSLIHA KGAYITTVGD YVGNYKKDVF DSWDNPSANA RKMFGSMLWS YDYSHFYFDP NIKIIPKKND WIHECGKLLN EGVVDCVVDK VYSWKNFKEA FSYMATQRAQ GKLIMKVEGF
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AST2 recombinant protein has garnered significant attention in the field of molecular biology and biotechnology due to its potential applications in therapeutic and diagnostic contexts. Astaxanthin, a notable carotenoid with strong antioxidant properties, is produced by various microorganisms, and the AST2 gene is critical in the biosynthesis of this compound. Researchers have focused on the expression and purification of AST2 recombinant protein to better understand its biochemical properties and interactions. Recombinant proteins like AST2 are instrumental in elucidating the enzymatic pathways involved in astaxanthin production, as well as exploring their functional roles in cellular processes. Furthermore, understanding the structure-function relationship of the AST2 protein can facilitate the development of biotechnological applications, such as synthesizing astaxanthin in a controlled environment or enhancing its yield in microbial production systems. Additionally, due to its potent antioxidant capabilities, AST2 and its derivative products are being investigated for potential health benefits, including their role in mitigating oxidative stress-related diseases. The study of AST2 recombinant protein thus represents a convergence of genetic engineering, metabolic pathway optimization, and health science, highlighting its relevance in advancing both fundamental research and commercial bioproduct development.
