Analytical Data
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基因名
ASNS
- Application
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别名
ASNS；TS11；Asparagine synthetase [glutamine-hydrolyzing]
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P08243
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表达区间
1-561aa
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氨基酸序列
MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRL AVVDPLFGMQPIRVKKYPYLWLCYNGEIYNHKKMQQHFEFEYQTKVDGEI ILHLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMT EDGFLAVCSEAKGLVTLKHSATPFLKVEPFLPGHYEVLDLKPNGKVASVE MVKYHHCRDEPLHALYDNVEKLFPGFEIETVKNNLRILFNNAVKKRLMTD RRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDSPDLLAAR KVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISK YIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESERLLRELYLF DVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRE TFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMAN AAQKFPFNTPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSAR TLTHYKSAVKA
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分子量
91 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
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Protein Description
ASNS (asparagine synthetase) is an essential enzyme involved in the biosynthesis of the amino acid asparagine, playing a crucial role in various physiological processes such as protein synthesis and cellular stress response. Research into ASNS has gained prominence in recent years due to its implications in cancer metabolism, particularly in tumors with high asparagine dependency, like certain types of leukemia. Inhibition of ASNS has been explored as a potential therapeutic strategy, as it may enhance the efficacy of asparaginase, a common treatment for acute lymphoblastic leukemia. The enzyme's role in regulating the cellular levels of asparagine has made it a focal point in studies aiming to understand cancer cell survival mechanisms in nutrient-deprived environments. Additionally, ASNS is involved in the metabolism of glutamine, further complicating its role in cancer biology. Recent structural studies have provided insights into the catalytic mechanism and substrate specificity of ASNS, paving the way for the design of targeted inhibitors that could improve clinical outcomes. Overall, continued research on ASNS could contribute significantly to developing novel anti-cancer therapies and enhancing our understanding of metabolic pathways in malignant cells.
