Analytical Data
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Gene name
PYGB
- Application
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Alternative Names
PYGB；Glycogen phosphorylase. brain form
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P11216
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Expression Region
477-731aa
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AA Sequence
PEKF QNKTNGITPR RWLLLCNPGL ADTIVEKIGE EFLTDLSQLK KLLPLVSDEV FIRDVAKVKQ ENKLKFSAFL EKEYKVKINP SSMFDVHVKR IHEYKRQLLN CLHVVTLYNR IKRDPAKAFV PRTVMIGGKA APGYHMAKLI IKLVTSIGDV VNHDPVVGDR LKVIFLENYR VSLAEKVIPA ADLSQQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM AEEAGAENLF IFGLRVEDVE ALDRKGYNAR E
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Molecular Weight
32 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PYGB (glycogen phosphorylase, brain form) is an enzyme that plays a pivotal role in glycogen metabolism, particularly in the brain and other tissues. The study of PYGB recombinant protein has garnered attention due to its central function in mobilizing glucose from glycogen stores, which is crucial for maintaining energy homeostasis, especially in neurons. Its dysregulation has been linked to various neurological disorders and metabolic diseases, making PYGB a potential therapeutic target. Given its importance in energy metabolism, researchers are interested in the biochemical characterization of PYGB, investigating its enzymatic activity, structure-function relationships, and regulatory mechanisms. Producing recombinant PYGB enables detailed studies on its properties and interactions with other cellular components. Techniques such as site-directed mutagenesis and structural biology approaches, including X-ray crystallography and nuclear magnetic resonance (NMR), are commonly employed to elucidate its function at a molecular level. Furthermore, understanding PYGB’s role in glycogen metabolism may offer insights into conditions like Alzheimer's disease and diabetes. As such, the research into PYGB recombinant protein not only enhances the understanding of fundamental metabolic processes but also opens avenues for developing novel therapeutic strategies targeting glycogen-related abnormalities.
