Analytical Data
-
Gene name
BFIT
- Application
-
Alternative Names
BFIT；BFIT；KIAA0707；THEA；Acyl-coenzyme A thioesterase 11
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q8WXI4
-
Expression Region
19-250aa
-
AA Sequence
MIQNVGNHLR RGLASVFSNR TSRKSALRAG NDSAMADGEG YRNPTEVQMS QLVLPCHTNQ RGELSVGQLL KWIDTTACLS AERHAGCPCV TASMDDIYFE HTISVGQVVN IKAKVNRAFN SSMEVGIQVA SEDLCSEKQW NVCKALATFV ARREITKVKL KQITPRTEEE KMEHSVAAER RRMRLVYADT IKDLLANCAI QGDLESRDCS RMVPAEKTRV ESVELVLPPH ANHQGNTFGG QIMAWMENVA TIAASRLCRA HPTLKAIEMF HFRGPSQVGD RLVLKAIVNN AFKHSMEVGV CVEAYRQEAE THRRHINSAF MTFVVLDADD QPQLLPWIRP QPGDGERRYR EASARKKIRL DRKYIVSCKQ TEVPLSVPWD PSNQVYLSYN NVSSLKMLVA KDNWVLSSEI SQVRLYTLED DKFLSFHMEM VVHVDAAQAF LLLSDLRQRP EWDKHYRSVE LVQQVDEDDA IYHVTSPALG GHTKPQDFVI LASRRKPCDN GDPYVIALRS VTLPTHRETP EYRRGETLCS GFCLWREGDQ LTKCCWVRVS LTELVSASGF YSWGLESRSK GRRSDGWNGK LAGGHLSTLK AIPVAKINSR FGYLQDT
-
Molecular Weight
30 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BFIT (Binding and Folding Intelligent Technology) recombinant protein has garnered significant attention in the field of protein engineering due to its potential applications in biotechnology and medicine. As the demand for highly specific and efficient proteins increases, researchers are exploring the versatility of recombinant proteins to address various challenges in drug development, enzyme engineering, and therapeutic interventions. The BFIT approach leverages advanced computational methods and high-throughput screening to optimize protein binding and folding characteristics, ensuring stability and functionality in diverse environments. This technology enables the design of proteins with tailored properties, enhancing their efficacy in targeted applications, such as targeting disease markers or improving the specificity of therapeutic agents. Furthermore, the study of BFIT recombinant proteins contributes to our understanding of protein dynamics and interactions, providing insights that can inform the design of novel biomolecules. As research progresses, the integration of BFIT strategies with emerging technologies, such as synthetic biology and CRISPR, holds the promise of revolutionizing the development of proteins that can meet the complex demands of modern biotherapeutics, ultimately leading to more effective treatments and improved patient outcomes.
