Analytical Data
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Gene name
HUWE1
- Application
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Alternative Names
(ARF-binding protein 1)(ARF-BP1)(HECT, UBA and WWE domain-containing protein 1)(HECT-type E3 ubiquitin transferase HUWE1)(Homologous to E6AP carboxyl terminus homologous protein 9)(HectH9)(Large structure of UREB1)(LASU1)(Mcl-1 ubiquitin ligase E3)(Mule)(Upstream regulatory element-binding protein 1)(URE-B1)(URE-binding protein 1)
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z6Z7
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Expression Region
4005-4374aa
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Molecular Weight
50.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HUWE1, also known as HEU1 or ARIH1, is a member of the RBR E3 ubiquitin ligase family and plays a crucial role in various cellular processes, including protein degradation, cell cycle regulation, and DNA damage response. The study of HUWE1 is of significant importance due to its involvement in the regulation of tumor suppressor proteins and oncogenes, making it a potential target for cancer therapy. Previous research has demonstrated that HUWE1 is overexpressed in several types of cancers, suggesting that it may contribute to tumorigenesis through the modulation of key signaling pathways. Furthermore, HUWE1 has been implicated in neurodegenerative diseases, highlighting its relevance in diverse biological contexts. The characterization of HUWE1, particularly through the expression of recombinant proteins, allows researchers to investigate its molecular mechanisms and interactions with substrates. Understanding the structure and function of HUWE1 can provide insights into its roles in health and disease, paving the way for potential therapeutic interventions. As such, the recombinant expression and study of HUWE1 are critical for elucidating its biological significance and potential applications in medicine.
