Analytical Data
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基因名
ME3
- Application
-
别名
Malic enzyme 3
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种属
Human
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表达系统
E. coli
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标签
N- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q16798
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表达区间
24-590aa
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分子量
67.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ME3, or Malic Enzyme 3, is an important enzyme that plays a critical role in cellular metabolism by catalyzing the conversion of malate to pyruvate while reducing NADP+ to NADPH. Its function is integral to various metabolic processes, including the regulation of the tricarboxylic acid (TCA) cycle and fatty acid synthesis. Research into ME3 has gained traction due to its potential implications in metabolism-related diseases, cancer, and obesity. Recent studies have highlighted the enzyme's role in tumor metabolism, suggesting that ME3 may be involved in the adaption of cancer cells to hypoxic environments and their overall growth. Understanding the mechanism of ME3, including its structure, regulation, and interaction with other metabolic pathways, can provide insights into novel therapeutic strategies. Scientists are increasingly focused on recombinant protein techniques to produce and purify ME3 for detailed biochemical analysis. Such studies aim to elucidate the enzyme's kinetic properties, substrate specificity, and potential post-translational modifications. Insights gained from ME3 research could reveal significant advancements in metabolic engineering and therapeutic development, ultimately contributing to the management of metabolic disorders and cancer treatments.
