Analytical Data
-
Gene name
SPG3A/ATL1
-
简介
The SPG3A/ATL1 protein is a GTPase that promotes membrane tethering by forming trans homomers, thereby achieving homotypic fusion of the endoplasmic reticulum (ER) membrane. It makes a crucial contribution to the biogenesis of the endoplasmic reticulum tubular network and shaping the structural organization of cells. SPG3A/ATL1 Protein, Human (sf9, His) is the recombinant human-derived SPG3A/ATL1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
-
Alternative Names
Atlastin-1; GTP-binding protein 3; GBP-3; ATL1; SPG3A
-
Species
Human
-
Source
Baculovirus
-
Tag
N-10*His
-
Purity
Greater than 95% as determined by SDS-PAGE.
-
Uniprot
Q8WXF7-1
-
Expression Region
M1-T447
-
AA Sequence
MAKNRRDRNSWGGFSEKTYEWSSEEEEPVKKAGPVQVLIVKDDHSFELDETALNRILLSEAVRDKEVVAVSVAGAFRKGKSFLMDFMLRYMYNQESVDWVGDYNEPLTGFSWRGGSERETTGIQIWSEIFLINKPDGKKVAVLLMDTQGTFDSQSTLRDSATVFALSTMISSIQVYNLSQNVQEDDLQHLQLFTEYGRLAMEETFLKPFQSLIFLVRDWSFPYEFSYGADGGAKFLEKRLKVSGNQHEELQNVRKHIHSCFTNISCFLLPHPGLKVATNPNFDGKLKEIDDEFIKNLKILIPWLLSPESLDIKEINGNKITCRGLVEYFKAYIKIYQGEELPHPKSMLQATAEANNLAAVATAKDTYNKKMEEICGGDKPFLAPNDLQTKHLQLKEESVKLFRGVKKMGGEEFSRRYLQQLESEIDELYIQYIKHNDSKNIFHAAR
-
Protein Length
Partial
-
Molecular Weight
54 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPG3A and ATL1 are proteins implicated in hereditary spastic paraplegia (HSP), a group of neurodegenerative disorders characterized by progressive weakness and stiffness of the legs. SPG3A, associated with the SPG3A form of HSP, is encoded by the SPAST gene, which plays a critical role in the regulation of microtubule dynamics, an essential process for axonal transport and neuronal function. ATL1, on the other hand, is linked to a different subtype of HSP and is known to be involved in endoplasmic reticulum (ER) function and stress response. Research on recombinant forms of these proteins has gained prominence in understanding the molecular mechanisms underlying their pathophysiology. By producing and characterizing SPG3A and ATL1 recombinant proteins, researchers aim to elucidate their roles in cellular processes, identify potential pathogenic variants, and develop therapeutic strategies. Furthermore, studying these proteins in a recombinant format allows for high-throughput screening of potential drugs and the exploration of interactions with other cellular components, ultimately contributing to the development of targeted treatments for HSP and related disorders. Overall, the investigation of SPG3A and ATL1 proteins not only deepens our understanding of HSP but also offers a promising avenue for therapeutic intervention.
