Analytical Data
-
Gene name
GCLM
- Application
-
Alternative Names
GCLM；GLCLR;Glutamate--cysteine ligase regulatory subunit
-
Species
Human
-
Source
E. coli
-
Tag
N-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P48507
-
Expression Region
2-274aa (K34Q)
-
AA Sequence
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSGTDSRAAKALLARARTLHLQTGNLLNWGRLRKQCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS
-
Molecular Weight
34 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
GCLM, or Glutamate-Cysteine Ligase Modifier Subunit, plays a critical role in the regulation of glutathione synthesis, which is essential for cellular antioxidant defense and detoxification processes. The enzyme complex, consisting of a catalytic subunit (GCLC) and a modifier subunit (GCLM), is pivotal in maintaining cellular redox balance and protecting against oxidative stress. Research into GCLM recombinant proteins has gained momentum due to its implications in various pathological conditions, including neurodegenerative diseases, cancer, and metabolic disorders. Understanding the function and regulation of GCLM can provide insights into therapeutic strategies aimed at enhancing antioxidant defenses in disease states. Recombinant GCLM offers a tool for detailed biochemical studies, allowing researchers to unravel the mechanistic underpinnings of glutathione biosynthesis and its impact on cellular health. Enhanced production of GCLM through recombinant techniques has the potential to facilitate new therapeutic interventions that target oxidative stress-related diseases, emphasizing the importance of this protein in biomedical research.
