Analytical Data
-
Gene name
BRD7
- Application
-
Alternative Names
75 kDa bromodomain Protein; BP75; BRD 7; BRD7; BRD7_HUMAN; Bromodomain containing 7
-
Species
Human
-
Source
E. coli
-
Tag
N-terminal His and SUMO Tag
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9NPI1
-
Expression Region
1-651aa
-
AA Sequence
MGKKHKKHKSDKHLYEEYVEKPLKLVLKVGGNEVTELSTGSSGHDSSLFEDKNDHDKHKDRKRKKRKKGEKQIPGEEKGRKRRRVKEDKKKRDRDRVENEAEKDLQCHAPVRLDLPPEKPLTSSLAKQEEVEQTPLQEALNQLMRQLQRKDPSAFFSFPVTDFIAPGYSMIIKHPMDFSTMKEKIKNNDYQSIEELKDNFKLMCTNAMIYNKPETIYYKAAKKLLHSGMKILSQERIQSLKQSIDFMADLQKTRKQKDGTDTSQSGEDGGCWQREREDSGDAEAHAFKSPSKENKKKDKDMLEDKFKSNNLEREQEQLDRIVKESGGKLTRRLVNSQCEFERRKPDGTTTLGLLHPVDPIVGEPGYCPVRLGMTTGRLQSGVNTLQGFKEDKRNKVTPVLYLNYGPYSSYAPHYDSTFANISKDDSDLIYSTYGEDSDLPSDFSIHEFLATCQDYPYVMADSLLDVLTKGGHSRTLQEMEMSLPEDEGHTRTLDTAKEMEITEVEPPGRLDSSTQDRLIALKAVTNFGVPVEVFDSEEAEIFQKKLDETTRLLRELQEAQNERLSTRPPPNMICLLGPSYREMHLAEQVTNNLKELAQQVTPGDIVSTYGVRKAMGISIPSPVMENNFVDLTEDTEEPKKTDVAECGPGGS
-
Molecular Weight
80.2kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
BRD7 is a member of the bromodomain and extraterminal (BET) family of proteins, which play a crucial role in regulating gene expression and chromatin dynamics through their interaction with acetylated lysine residues on histones and various transcription factors. Research has increasingly highlighted the significance of BRD7 in several biological processes, including cell cycle regulation, apoptosis, and differentiation. Dysregulation of BRD7 has been implicated in various cancers, where it may act as a tumor suppressor by modulating pathways involved in cell proliferation and survival. Recent studies have focused on the reconstitution of BRD7 into model systems to dissect its functional roles and potential mechanisms of action at the molecular level. Understanding the structure-function relationship of BRD7, along with its interactions with other proteins and chromatin, is essential for elucidating its contributions to cellular homeostasis and malignancy. Given the therapeutic potential of targeting BET family proteins in cancer treatment, the study of BRD7 can provide insights not only for cancer biology but also for the development of novel therapeutic strategies. Current research is exploring the recombinant production of BRD7 to facilitate these investigations, enabling detailed analysis of its biophysical properties, functional interactions, and therapeutic implications.
