Analytical Data
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基因名
APOBEC3B
- Application
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别名
APOBEC3BDNA dC->dU-editing enzyme APOBEC-3B; A3B; EC 3.5.4.38; Phorbolin-1-related Protein; Phorbolin-2/3
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种属
Human
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表达系统
E. coli
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标签
N-terminal His Tag
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UH17
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表达区间
1-382aa
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氨基酸序列
MNPQIRNPMERMYRDTFYDNFENEPILYGRSYTWLCYEVKIKRGRSNLLWDTGV FRGQVYFKPQYHAEMCFLSWFCGNQLPAYKCFQITWFVSWTPCPDCVAKLAEFL SEHPNVTLTISAARLYYYWERDYRRALCRLSQAGARVTIMDYEEFAYCWENFVY NEGQQFMPWYKFDENYAFLHRTLKEILRYLMDPDTFTFNFNNDPLVLRRRQTYL CYEVERLDNGTWVLMDQHMGFLCNEAKNLLCGFYGRHAELRFLDLVPSLQLDPA QIYRVTWFISWSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQ MLRDAGAQVSIMTYDEFEYCWDTFVYRQGCPFQPWDGLEEHSQALSGRLRAILQ NQGN
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分子量
49.3kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
APOBEC3B is a member of the APOBEC (Apolipoprotein B mRNA Editing Enzyme Catalytic Polypeptide) family, which is known for its role in regulating RNA and DNA editing. Its primary function involves deaminating cytosine residues in single-stranded DNA, leading to mutations that can hinder viral replication and contribute to genomic diversity. A critical aspect of APOBEC3B research is its implication in cancer biology; overexpression of this enzyme has been linked to the accumulation of mutations in tumor DNA, thereby influencing tumorigenesis and cancer progression. Understanding the structural and functional characteristics of APOBEC3B, including its protein-protein interactions and regulatory mechanisms, is vital for elucidating its role in both antiviral defense and oncogenesis. Additionally, studies have indicated that this enzyme's activity can be modulated by various cellular factors, which positions it as a potential target for therapeutic interventions aimed at controlling viral infections or cancer development. The ongoing exploration of APOBEC3B, including the design and testing of recombinant proteins, aims to enhance our understanding of its diverse functions and pave the way for novel strategies in molecular medicine.
