Analytical Data
-
Gene name
GLRA3
- Application
-
Alternative Names
GLRA 3; Glra3; GLRA3_HUMAN; Glycine receptor alpha 3 polypeptide; Glycine receptor alpha 3 subunit; Glycine receptor subunit alpha 3; Glycine receptor subunit alpha-3; Ligand gated ion channel
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O75311
-
Expression Region
34-464aa
-
AA Sequence
ARSRSAPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSIAETTMDYRVNIFLRQKWNDPRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGANFHEVTTDNKLLRIFKNGNVLYSIRLTLTLSCPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQDEAPVQVAEGLTLPQFLLKEEKDLRYCTKHYNTGKFTCIEVRFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRKNKTEAFALEKFYRFSDMDDEVRESRFSFTAYGMGPCLQAKDGMTPKGPNHPVQVMPKSPDEMRKVFIDRAKKIDTISRACFPLAFLIFNIFYWVIYKILRHEDIHQQQD
-
Molecular Weight
73.15 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GLRA3, or Glycine Receptor Alpha Subunit 3, is a member of the ionotropic glycine receptor family, which plays a crucial role in inhibitory neurotransmission within the central nervous system. It primarily mediates synaptic inhibition by allowing chloride ions to flow into neurons upon binding glycine, thus calming excessive neuronal activity. Mutations or dysregulation of GLRA3 have been implicated in various neurological disorders, including certain forms of epilepsy, schizophrenia, and movement disorders. Research into the recombinant expression of GLRA3 protein is vital for understanding its functional properties, interaction with ligands, and the mechanisms underlying its involvement in these pathological conditions. The ability to produce and purify GLRA3 in a laboratory setting facilitates structural and biophysical studies, which are essential for developing therapeutic strategies aimed at modulating glycinergic signaling. Furthermore, advances in recombinant technology have opened avenues for exploring the receptor's pharmacological profiles and identification of potential drug targets, thus paving the way for novel treatments for related neurophysiological disorders. The investigation of GLRA3 not only contributes to our understanding of glycinergic neurotransmission but also highlights its significance in neuropharmacology and potential clinical applications.
