Analytical Data
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Gene name
GALK2
- Application
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Alternative Names
GALK2; GaINAc Kinase; Galactokinase 2; Galk2; GALK2_HUMAN; GalNAc kinase; GK2; MGC1745; N-acetylgalactosamine kinase
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q01415
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Expression Region
1-458aa
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AA Sequence
MATESPATRRVQVAEHPRLLKLKEMFNSKFGSIPKFYVRAPGRVNIIGEHIDYCGYSVLPMAVEQDVLIAVEPVKTYALQLANTNPLYPDFSTSANNIQIDKTKPLWHNYFLCGLKGIQEHFGLSNLTGMNCLVDGNIPPSSGLSSSSALVCCAGLVTLTVLGRNLSKVELAEICAKSERYIGTEGGGMDQSISFLAEEGTAKLIEFSPLRATDVKLPSGAVFVIANSCVEMNKAATSHFNIRVMECRLAAKLLAKYKSLQWDKVLRLEEVQAKLGISLEEMLLVTEDALHPEPYNPEEICRCLGISLEELRTQILSPNTQDVLIFKLYQRAKHVYSEAARVLQFKKICEEAPENMVQLLGELMNQSHMSCRDMYECSCPELDQLVDICRKFGAQGSRLTGAGWGGCTVSMVPADKLPSFLANVHKAYYQRSDGSLAPEKQSLFATKPGGGALVLLEA
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Molecular Weight
76.01 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GALK2, or galactokinase 2, is an enzyme that plays a crucial role in the metabolism of galactose, a sugar found in various dietary sources. It catalyzes the phosphorylation of galactose to galactose-1-phosphate, a key intermediate in the Leloir pathway of galactose metabolism. Research on GALK2 is particularly important due to its implications in human health, as deficiencies in galactokinase can lead to galactosemia, a genetic disorder characterized by the inability to metabolize galactose, resulting in increased galactose levels in the body and potential developmental, neurological, and liver issues. Understanding GALK2, especially in terms of its structure and function, is vital for developing therapeutic strategies and interventions for galactosemia and related metabolic disorders. Recent advances in recombinant protein technology allow for the expression and purification of GALK2, enabling researchers to conduct detailed biochemical studies and structural analyses. These studies can provide insights into the enzyme's catalytic mechanism, substrate specificity, and regulatory aspects, paving the way for potential drug designs that may mitigate the effects of galactosemia and enhance our understanding of carbohydrate metabolism. Thus, the recombinant production of GALK2 not only facilitates basic research but also holds promise for clinical applications in precision medicine, making it a significant focus within the fields of biochemistry and metabolic disease research.
