Analytical Data
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基因名
GAK
- Application
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别名
Auxilin 2; Cyclin G associated kinase; Cyclin-G-associated kinase; DNAJ26; DNAJC26; EC 2.7.11.1; FLJ16629; FLJ40395; GAK; GAK protein; GAK_HUMAN; MGC99654
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O14976
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表达区间
24-416aa
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氨基酸序列
DQSDFVGQTVELGELRLLLASPAPPLSVQSTPRGGPPAAADPFGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSSPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTIAEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAPEQVKKHYRRAVLAVHPDKAAGQPYEQHAKMIFMELNDAWSEFENQGSRPLF
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分子量
68.97 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GAK, or cyclin G-associated kinase, is a serine/threonine kinase that plays a crucial role in various cellular processes, including cell cycle regulation, endocytosis, and neuronal function. The understanding of GAK's functions has grown significantly due to its association with several diseases, particularly cancer and neurodegenerative disorders. Researchers have been particularly interested in the reconstituted GAK protein because it can help elucidate the mechanisms underlying its involvement in signaling pathways and cellular processes. The study of GAK reconstitution has enabled scientists to analyze its enzymatic activity, protein-protein interactions, and regulatory mechanisms in a more controlled environment, thus providing insights into its biological roles. Furthermore, GAK is implicated in the regulation of Clathrin-mediated endocytosis, a critical process for cellular uptake of nutrients and signaling molecules. Understanding GAK's structure and function through recombinant protein studies can reveal potential therapeutic targets for diseases where GAK dysregulation is observed. As such, the research on GAK reconstituted proteins stands at the intersection of molecular biology and therapeutic development, contributing significantly to our understanding of cell biology and its implications in health and disease.
