Analytical Data
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Gene name
GAK
- Application
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Alternative Names
Auxilin 2; Cyclin G associated kinase; Cyclin-G-associated kinase; DNAJ26; DNAJC26; EC 2.7.11.1; FLJ16629; FLJ40395; GAK; GAK protein; GAK_HUMAN; MGC99654
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14976
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Expression Region
24-416aa
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AA Sequence
DQSDFVGQTVELGELRLLLASPAPPLSVQSTPRGGPPAAADPFGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSSPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTIAEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAPEQVKKHYRRAVLAVHPDKAAGQPYEQHAKMIFMELNDAWSEFENQGSRPLF
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Molecular Weight
68.97 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GAK, or cyclin G-associated kinase, is a serine/threonine kinase that plays a crucial role in various cellular processes, including cell cycle regulation, endocytosis, and neuronal function. The understanding of GAK's functions has grown significantly due to its association with several diseases, particularly cancer and neurodegenerative disorders. Researchers have been particularly interested in the reconstituted GAK protein because it can help elucidate the mechanisms underlying its involvement in signaling pathways and cellular processes. The study of GAK reconstitution has enabled scientists to analyze its enzymatic activity, protein-protein interactions, and regulatory mechanisms in a more controlled environment, thus providing insights into its biological roles. Furthermore, GAK is implicated in the regulation of Clathrin-mediated endocytosis, a critical process for cellular uptake of nutrients and signaling molecules. Understanding GAK's structure and function through recombinant protein studies can reveal potential therapeutic targets for diseases where GAK dysregulation is observed. As such, the research on GAK reconstituted proteins stands at the intersection of molecular biology and therapeutic development, contributing significantly to our understanding of cell biology and its implications in health and disease.
