Analytical Data
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Gene name
GAL3ST4
- Application
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Alternative Names
GAL3ST4; PP6968; Galactose-3-O-sulfotransferase 4; Gal3ST-4; EC 2.8.2.-; Beta-galactose-3-O-sulfotransferase 4; Gal-beta-1.3-GalNAc 3'-sulfotransferase
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96RP7
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Expression Region
1-486aa
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AA Sequence
MGPLSPARTLRLWGPRSLGVALGVFMTIGFALQLLGGPFQRRLPGLQLRQPSAPSLRPALPSCPPRQRLVFLKTHKSGSSSVLSLLHRYGDQHGLRFALPARYQFGYPKLFQASRVKGYRPQGGGTQLPFHILCHHMRFNLKEVLQVMPSDSFFFSIVRDPAALARSAFSYYKSTSSAFRKSPSLAAFLANPRGFYRPGARGDHYARNLLWFDFGLPFPPEKRAKRGNIHPPRDPNPPQLQVLPSGAGPRAQTLNPNALIHPVSTVTDHRSQISSPASFDLGSSSFIQWGLAWLDSVFDLVMVAEYFDESLVLLADALCWGLDDVVGFMHNAQAGHKQGLSTVSNSGLTAEDRQLTARARAWNNLDWALYVHFNRSLWARIEKYGQGRLQTAVAELRARREALAKHCLVGGEASDPKYITDRRFRPFQFGSAKVLGYILRSGLSPQDQEECERLATPELQYKDKLDVKQFPPTVSLPLKTSRPLSP
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Molecular Weight
80.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GAL3ST4, or Galactose-3-O-sulfotransferase 4, is an important enzyme involved in the sulfation of glycosaminoglycans, particularly heparan sulfate, which plays a crucial role in various biological processes including cell signaling, development, and extracellular matrix organization. Aberrant sulfation patterns have been implicated in numerous pathologies, including cancer and genetic disorders. The recombinant expression of GAL3ST4 has become a focal point of research due to its potential applications in understanding these disease mechanisms and developing new therapeutic strategies. By producing this protein in a controlled system, researchers aim to elucidate its biochemical properties, enzymatic activity, and interaction with substrates. Studies of GAL3ST4 could provide insights into the regulatory mechanisms of sulfation and its impact on cellular functions. Additionally, understanding the structure-function relationship of GAL3ST4 may inform the design of specific inhibitors or modulators, paving the way for innovative treatment options for conditions related to abnormal glycosaminoglycan metabolism. As such, GAL3ST4 stands at the intersection of enzymology, cell biology, and therapeutic development, marking it as a significant target for further investigation.
