Cat: PA2000-7835

Recombinant Human FRAT1 Protein,GST

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Analytical Data

  • Gene name

    FRAT1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    FRAT1Proto-oncogene FRAT1; Frequently rearranged in advanced T-cell lymphomas 1; FRAT-1

  • Species

    Human

  • Source

    E. coli

  • Tag

    GST-tag at N-terminal

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q92837

  • Expression Region

    1-279aa

  • AA Sequence

    MPCRREEEEE AGEEAEGEEE EEDSFLLLQQ SVALGSSGEV DRLVAQIGET LQLDAAQHSP ASPCGPPGAP LRAPGPLAAA VPADKARSPA VPLLLPPALA ETVGPAPPGV LRCALGDRGR VRGRAAPYCV AELATGPSAL SPLPPQADLD GPPGAGKQGI PQPLSGPCRR GWLRGAAASR RLQQRRGSQP ETRTGDDDPH RLLQQLVLSG NLIKEAVRRL HSRRLQLRAK LPQRPLLGPL SAPVHEPPSP RSPRAACSDP GASGRAQLRT GDGVLVPGS

  • Molecular Weight

    29.0 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

FRAT1 (Frat Associated Protein 1) is a crucial component in the Wnt signaling pathway, which plays a significant role in cell proliferation, differentiation, and development. Dysregulation of this pathway has been implicated in various diseases, including cancer, making FRAT1 a potential target for therapeutic intervention. Research has shown that FRAT1 functions as a key regulator, stabilizing β-catenin and promoting Wnt target gene expression. The understanding of FRAT1's structure and interactions at the molecular level is essential for developing novel strategies to modulate its activity. Recombinant FRAT1 proteins are crucial for studying its biological functions, interactions with other proteins, and potential as a drug target. Producing and characterizing FRAT1 recombinant proteins enables researchers to investigate its role in pathological conditions and assess the effects of small molecules on Wnt signaling. Additionally, gaining insights into the mechanisms of FRAT1’s action may provide valuable information for designing inhibitors or activators that could be utilized in clinical settings. Continued research on FRAT1 and its associated pathways holds promise for innovative approaches in cancer therapy and regenerative medicine.

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