Analytical Data
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基因名
PPOX
- Application
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别名
PPOX；Protoporphyrinogen oxidase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P50336
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表达区间
1-477aa
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氨基酸序列
MGRTVVVLGGGISGLAASYHLSRAPCPPKVVLVESSERLGGWIRSVRGPNGAIFELGPRGIRPAGALGARTLLLVSELGLDSEVLPVRGDHPAAQNRFLYVGGALHALPTGLRGLLRPSPPFSKPLFWAGLRELTKPRGKEPDETVHSFAQRRLGPEVASLAMDSLCRGVFAGNSRELSIRSCFPSLFQAEQTHRSILLGLLLGAGRTPQPDSALIRQALAERWSQWSLRGGLEMLPQALETHLTSRGVSVLRGQPVCGLSLQAEGRWKVSLRDSSLEADHVISAIPASVLSELLPAEAAPLARALSAITAVSVAVVNLQYQGAHLPVQGFGHLVPSSEDPGVLGIVYDSVAFPEQDGSPPGLRVTVMLGGSWLQTLEASGCVLSQELFQQRAQEAAATQLGLKEMPSHCLVHLHKNCIPQYTLGHWQKLESARQFLTAHRLPLTLAGASYEGVAVNDCIESGRQAAVSVLGTEPNS
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分子量
66.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPOX, or protoporphyrinogen oxidase, is an essential enzyme in the heme biosynthesis pathway, catalyzing the conversion of protoporphyrinogen IX to protoporphyrin IX. Mutations in the PPOX gene are linked to variegate porphyria, a genetic disorder that leads to the accumulation of porphyrins, resulting in neurological and cutaneous symptoms. Given the critical role of PPOX in heme production and metabolic regulation, research into this enzyme has significant implications for understanding porphyrias and related disorders. Additionally, as heme is a vital component for various hemoproteins, including hemoglobin and cytochromes, studying PPOX can unveil insights into broader biological processes. Recombinant PPOX production enables detailed characterization of the enzyme's structure, function, and kinetics while also providing a platform for potential therapeutic interventions. By using recombinant DNA technology, scientists can generate large quantities of PPOX for biochemical studies, explore its interaction with inhibitors, and assess the impact of specific mutations on enzyme activity. This line of research could lead to novel therapeutic strategies for managing porphyria and enhancing our understanding of heme biosynthesis, with broader applications in synthetic biology and drug development. As such, PPOX stands at the intersection of fundamental biochemical research and clinical relevance, underscoring the importance of continued studies in this area.
