Analytical Data
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Gene name
EIF4A2
- Application
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Alternative Names
EIF4A2；DDX2B；EIF4F；Eukaryotic initiation factor 4A-II
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14240
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Expression Region
1-407aa
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AA Sequence
MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI
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Molecular Weight
73.4kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF4A2, a member of the eukaryotic initiation factor 4A family, encodes a DEAD-box RNA helicase that is essential for the initiation of translation in eukaryotic cells. This protein plays a critical role in unwinding mRNA secondary structures, facilitating the binding of ribosomal subunits and the recruitment of other translation factors. Recent studies have highlighted the significance of EIF4A2 in various biological processes, including cell proliferation, differentiation, and response to stress. Dysregulation of this protein has been implicated in several diseases, particularly cancer, where EIF4A2 expression is often upregulated, contributing to the enhanced translation of oncogenic mRNAs. The recombinant production of EIF4A2 has emerged as a vital tool for understanding its biochemical properties, functional mechanisms, and interactions with other cellular components. By studying the recombinant protein, researchers aim to elucidate its role in translation regulation and explore potential therapeutic targets for diseases associated with its dysfunction. Through structural and functional analyses, the insights gained could pave the way for the development of novel strategies to modulate EIF4A2 activity, thereby providing a new avenue for cancer treatment and other related disorders.
