Cat: PA1000-8726

Recombinant Human EIF3A Protein,His

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Analytical Data

  • Gene name

    EIF3A

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    EIF3A;EIF3S10;KIAA0139;Eukaryotic translation initiation factor 3 subunit A

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P56537

  • Expression Region

    1-245aa

  • AA Sequence

    MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLPD TVQIRRVEER LSALGNVTTC NDYVALVHPD LDRETEEILA DVLKVEVFRQ TVADQVLVGS YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP LVAGTVNRGS EVIAAGMVVN DWCAFCGLDT TSTELSVVES VFKLNEAQPS TIATSMRDSL IDSLT

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

EIF3A, or Eukaryotic Translation Initiation Factor 3 Subunit A, is a critical component of the translation initiation machinery in eukaryotic cells. It plays a pivotal role in the formation of the translation initiation complex by interacting with various other initiation factors and the ribosome. Research into EIF3A has gained prominence due to its involvement in the regulation of gene expression and its potential implications in various diseases, particularly cancer. Abnormalities in EIF3A expression or function have been linked to tumorigenesis, making it a significant focus for cancer research. Additionally, EIF3A's role in cellular stress responses and its interaction with viral proteins highlight its importance in both normal cellular processes and viral pathogenesis. Recombinant EIF3A proteins are being studied for their potential applications in drug discovery and the development of therapeutic interventions. Understanding the structure and function of EIF3A through recombinant protein technology can provide insights into its mechanisms and interactions, paving the way for novel strategies to target its pathways in disease contexts. This research ultimately aims to elucidate the multifaceted roles of EIF3A in translation initiation and cellular regulation, contributing to the broader understanding of gene expression control in health and disease.

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