Analytical Data
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Gene name
CENPF
- Application
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Alternative Names
CENPF；Centromere Protein F
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49454
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Expression Region
1759-2093aa
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AA Sequence
AIQGRNESCDISKEHTSETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSPDTNYEPPGEDKTQGSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQELLQRVETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDVENELSRIRSEKASIEHEALYLEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTSERNQLRGELDTMSKKTTALDQLSEKMKEKTQELESHQSE
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Molecular Weight
82.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CENPF (Centromere Protein F) is a critical protein involved in the mitotic process, particularly in the maintenance of centromere integrity during cell division. Its role extends beyond mere structural support, as it is implicated in various cellular functions including chromosome segregation and the regulation of cell cycle progression. Given its importance in mitosis, aberrations in CENPF expression have been linked to several diseases, notably cancer, where it is often upregulated in various tumor types. This makes CENPF a potential marker for tumorigenesis and a candidate for targeted therapies. Research efforts have increasingly focused on the characterization and functional analysis of recombinant CENPF to better understand its mechanisms and interactions in cellular processes. The production of recombinant CENPF allows for detailed studies of its structural properties, functional roles, and interactions with other mitotic proteins. Furthermore, exploring the phosphorylation and post-translational modifications of CENPF can elucidate its regulatory mechanisms and involvement in disease pathways. This research not only aims to enhance our understanding of CENPF's fundamental biology but also seeks to explore its potential as a therapeutic target, paving the way for novel cancer treatments and improved patient outcomes. As such, studies on recombinant CENPF are critical in advancing our comprehension of cell division anomalies that lead to oncogenesis, thereby highlighting the intersection between basic research and clinical application.
