Analytical Data
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Gene name
CENPB
- Application
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Alternative Names
CENPB；Major centromere autoantigen B
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P07199
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Expression Region
2-136aa
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AA Sequence
GPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR AILASERKYG VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL KEKALRIAEE LGMDDFTASN GWLDRFRRRH GVVSCS
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Molecular Weight
19 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CENPB (Centromere Protein B) is a crucial protein involved in the assembly of the centromere, playing a key role in chromosome segregation during mitosis and meiosis. As a component of the centromere complex, CENPB is essential for the recruitment of other centromeric proteins, ensuring the proper formation of the kinetochore, which is critical for accurate chromosome alignment and separation. Dysregulation or mutations in the CENPB gene can lead to various chromosomal disorders and contribute to tumorigenesis, making it a significant target for cancer research. Recent studies have focused on the production of recombinant CENPB protein to investigate its detailed functional mechanisms and its interactions with other centromeric proteins. The availability of recombinant CENPB allows researchers to perform structural analyses, binding assays, and functional assays to better understand its role in centromere dynamics. Furthermore, characterizing the effects of post-translational modifications on CENPB function could provide insights into the regulatory mechanisms governing centromere activity. Overall, research on CENPB and its recombinant forms holds promise for advancing our understanding of chromosome biology and its implications in diseases, particularly cancer-related abnormalities associated with centromere malfunction.
