Analytical Data
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Gene name
LASS2
- Application
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Alternative Names
LASS2；LASS2；TMSG1；Ceramide synthase 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96G23
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Expression Region
1-380aa
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AA Sequence
LQTLYDYFWWERLWLPVNLTWADLEDRDGRVYAKASDLYITLPLALLFLIVRYFFELYVATPLAALLNIKEKTRLRAPPNATLEHFYLTSGKQPKQVEVELLSRQSGLSGRQVERWFRRRRNQDRPSLLKKFREASWRFTFYLIAFIAGMAVIVDKPWFYDMKKVWEGYPIQSTIPSQYWYYMIELSFYWSLLFSIASDVKRKDFKEQIIHHVATIILISFSWFANYIRAGTLIMALHDSSDYLLESAKMFNYAGWKNTCNNIFIVFAIVFIITRLVILPFWILHCTLVYPLELYPAFFGYYFFNSMMGVLQLLHIFWAYLILRMAHKFITGKLVEDERSDREETESSEGEEAAAGGGAKSRPLANGHPILNNNHRKND
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Molecular Weight
44.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LASS2 (Lipid Agnostic Sphingolipid Synthase 2), a member of the LASS family, plays a crucial role in the biosynthesis of sphingolipids, which are vital components of cell membranes and are involved in various cellular processes, including signaling, apoptosis, and cell differentiation. Research into LASS2 has gained attention due to its implications in various diseases, particularly cancer, where it has been linked to tumor progression and metastasis. The enzyme is responsible for synthesizing ceramide, a key sphingolipid that acts as a second messenger in cell signaling pathways. Alterations in LASS2 expression and activity have been associated with pathological conditions, highlighting its potential as a therapeutic target. Understanding the structural and functional properties of LASS2 is essential for developing targeted therapies that could modulate its activity. Consequently, the recombinant expression of LASS2 protein has become a significant focus in biochemical and pharmacological research, providing insights into its enzymatic mechanisms and interactions with other cellular components. This knowledge not only enhances our understanding of sphingolipid metabolism but also paves the way for innovative strategies in treating diseases linked to aberrant sphingolipid signaling. Researchers are employing various expression systems to produce LASS2 for biochemical characterization and functional assays, aiming to elucidate its role in health and disease. Overall, the study of LASS2 as a recombinant protein is pivotal in advancing our understanding of sphingolipid biology and its clinical implications.
