Analytical Data
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基因名
DHCR7
- Application
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别名
DHCR7；D7SR；7-dehydrocholesterol reductase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UBM7
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表达区间
1-475aa
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氨基酸序列
MAAKLQPNIPKAKSLDGVTNDRTASQGQWGRAWEVDWFSLASVIFLLLFA PFIVYYFIMACDQYSCALTGPVVDIVTGHARLSDIWAKTPPITRKAAQLY TLWVTFQVLLYTSLPDFCHKFLPGYVGGIQEGAVTPAGVVNKYQINGLQA WLLTHLLWFANAHLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMVKGY FFPTSARDCKFTGNFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTL INLSFAAKQRELHSHVTNAMVLVNVLQAIYVIDFFWNETWYLKTIDICHD HFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPHAVGVLLLGLVGYYI FRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFW GVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHR CASKYGRDWERYTAAVPYRLLPGIF
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DHCR7 (7-dehydrocholesterol reductase) is an essential enzyme involved in the cholesterol biosynthesis pathway, specifically catalyzing the conversion of 7-dehydrocholesterol to cholesterol. Mutations in the DHCR7 gene are linked to Smith-Lemli-Opitz syndrome (SLOS), a genetic disorder characterized by a range of developmental and physical abnormalities due to impaired cholesterol synthesis. The investigation of DHCR7 and its recombinant protein has gained prominence due to its vital role in cellular membrane integrity and signaling, as well as its implications in developmental biology and disease mechanisms. Researchers are particularly interested in the structure-function relationship of the DHCR7 protein, as understanding its dynamics may provide insights into therapeutic strategies for conditions stemming from cholesterol biosynthesis defects. Furthermore, recombinant DHCR7 can serve as a valuable tool in drug discovery and development, aiding in the creation of compounds that can either mimic its activity or regulate its function. By elucidating the enzymatic activity and regulatory mechanisms of DHCR7 through in vitro studies and structural analysis, scientists hope to uncover potential intervention points for treating SLOS and related cholesterol metabolism disorders. Overall, the study of DHCR7 recombinant protein stands at the intersection of basic biochemistry and clinical application, highlighting the enzyme's significance in both healthy physiology and disease pathology.
