Analytical Data
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Gene name
USP49
- Application
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Alternative Names
USP49; Ubiquitin carboxyl-terminal hydrolase 49; EC 3.4.19.12; Deubiquitinating enzyme 49; Ubiquitin thioesterase 49; Ubiquitin-specific-processing protease 49
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q70CQ1
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Expression Region
1-688 aa
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AA Sequence
MDRCKHVGRL RLAQDHSILN PQKWCCLECA TTESVWACLK CSHVACGRYI EDHALKHFEE TGHPLAMEVR DLYVFCYLCK DYVLNDNPEG DLKLLRSSLL AVRGQKQDTP VRRGRTLRSM ASGEDVVLPQ RAPQGQPQML TALWYRRQRL LARTLRLWFE KSSRGQAKLE QRRQEEALER KKEEARRRRR EVKRRLLEEL ASTPPRKSAR LLLHTPRDAG PAASRPAALP TSRRVPAATL KLRRQPAMAP GVTGLRNLGN TCYMNSILQV LSHLQKFREC FLNLDPSKTE HLFPKATNGK TQLSGKPTNS SATELSLRND RAEACEREGF CWNGRASISR SLELIQNKEP SSKHISLCRE LHTLFRVMWS GKWALVSPFA MLHSVWSLIP AFRGYDQQDA QEFLCELLHK VQQELESEGT TRRILIPFSQ RKLTKQVLKV VNTIFHGQLL SQVTCISCNY KSNTIEPFWD LSLEFPERYH CIEKGFVPLN QTECLLTEML AKFTETEALE GRIYACDQCN SKRRKSNPKP LVLSEARKQL MIYRLPQVLR LHLKRFRWSG RNHREKIGVH VVFDQVLTME PYCCRDMLSS LDKETFAYDL SAVVMHHGKG FGSGHYTAYC YNTEGGFWVH CNDSKLNVCS VEEVCKTQAY ILFYTQRTVQ GNARISETHL QAQVQSSNND EGRPQTFS
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Molecular Weight
79.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
USP49, a member of the ubiquitin-specific protease family, plays a crucial role in regulating protein degradation and cellular signaling pathways through deubiquitination. As an enzyme that removes ubiquitin chains from substrates, USP49 directly influences various biological processes, including cell cycle regulation, response to stress, and apoptosis. Recent studies have shown that USP49 is involved in key cellular functions such as maintaining proteostasis and modulating immune responses, highlighting its potential as a therapeutic target. Given its implications in various diseases, including cancer and neurodegenerative disorders, research on USP49 recombinant protein has gained significance. Scientists aim to elucidate its precise biological mechanisms, interactions with other proteins, and the regulatory networks it participates in. Understanding these aspects could provide insights into the development of innovative therapeutic strategies. Additionally, the production of USP49 recombinant protein facilitates functional studies, including enzymatic activity assays and structural analyses, further advancing our knowledge of this important regulator in cellular homeostasis and disease pathology.
