Analytical Data
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Gene name
HDAC2
- Application
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Alternative Names
HDAC2；Histone deacetylase 2
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Species
Human
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Source
E. coli
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Tag
N-terminal His-Tag and GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92769
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Expression Region
1-488aa
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AA Sequence
MAYSQGGGKKKVCYYYDGDIGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEI YRPHKATAEEMTKYHSDEYIKFLRSIRPDNMSEYSKQMQRFNVGEDCPVFDGLF EFCQLSTGGSVAGAVKLNRQQTDMAVNWAGGLHHAKKSEASGFCYVNDIVLAIL ELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIG AGKGKYYAVNFPMRDGIDDESYGQIFKPIISKVMEMYQPSAVVLQCGADSLSGD RLGCFNLTVKGHAKCVEVVKTFNLPLLMLGGGGYTIRNVARCWTYETAVALDCE IPNELPYNDYFEYFGPDFKLHISPSNMTNQNTPEYMEKIKQRLFENLRMLPHAP GVQMQAIPEDAVHEDSGDEDGEDPDKRISIRASDKRIACDEEFSDSEDEGEGGR RNVADHKKGAKKARIEEDKKETEDKKTDVKEEDKSKDNSGEKTDTKGTKSEQLS NP
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Molecular Weight
81.3kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
The study of HDAC2 (Histone Deacetylase 2) recombinant protein has gained significant attention due to its crucial role in various cellular processes, including gene expression regulation, cell differentiation, and apoptosis. HDAC2 is part of the histone deacetylase family, enzymes that remove acetyl groups from histones, leading to a compact chromatin structure and transcriptional repression. Dysregulation of HDAC2 has been implicated in several diseases, particularly cancer, neurodegenerative disorders, and cardiovascular diseases, making it a promising target for therapeutic intervention. Notably, HDAC2 has been associated with tumorigenesis, where its overexpression contributes to the silencing of tumor suppressor genes. The development of HDAC inhibitors has emerged as a potential strategy for reversing these effects and restoring normal gene expression. Furthermore, understanding the structure and function of recombinant HDAC2 can provide insights into its mechanisms of action and facilitate the design of specific inhibitors. Recent advances in protein engineering and purification techniques have enabled the production of functional recombinant HDAC2, allowing researchers to delve deeper into its biochemical properties and interactions with various substrates. This research is not only critical for elucidating HDAC2's role in health and disease but also for advancing the field of epigenetic therapies, highlighting the importance of this enzyme in potential clinical applications.
