Analytical Data
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基因名
ZPBP
- Application
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别名
Zona pellucida-binding protein 1. Inner acrosomal membrane IAM38. Sp38
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BS86
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表达区间
45-351 aa
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氨基酸序列
HLVRLPRAFRLTKDSVKIVGSTSFPVKAYVMLHQKSPHVLCVTQQLRNAELIDPSFQWYGPKGKVVSVENRTAQITSTGSLVFQNFEESMSGIYTCFLEYKPTVEEIVKRLQLKYAIYAYREPHYYYQFTARYHAAPCNSIYNISFEKKLLQILSKLLLDLSCEISLLKSECHRVKMQRAGLQNELFFAFSVSSLDTEKGPKRCTDHNCEPYKRLFKAKNLIERFFNQQVEILGRRAEQLPQIYYIEGTLQMVWINRCFPGYGMNVQQHPKCPECCVICSPGSYNPRDGIHCLQCNSSLVYGAKTCL
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分子量
59.51 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ZPBP (Zonadhesin-like Protein Binding Protein) is a critical protein that has garnered significant attention in the field of reproductive biology and biochemistry due to its role in gamete recognition and fertilization. Its study is particularly important in understanding the mechanisms of sperm-egg interaction, which is essential for successful fertilization. ZPBP is hypothesized to interact with zona pellucida proteins present on the egg surface, facilitating binding and subsequent fusion of sperm and egg. The exploration of ZPBP encompasses various areas such as molecular biology, genetics, and reproductive technology. This research is vital not only for elucidating the fundamental processes of mammalian reproduction but also for the development of new contraceptives and treatments for infertility. Advances in recombinant protein technology have enabled the production of ZPBP for in-depth functional studies, opening avenues for investigating its structural properties and biological functions. Furthermore, understanding the expression patterns and regulatory mechanisms of ZPBP can provide insights into species-specific fertilization processes and contribute to the understanding of reproductive disorders. As such, ZPBP research represents a significant intersection of basic science and applied reproductive medicine, with implications for both human health and agricultural biotechnology. The ongoing research in this area aims to enhance our knowledge of reproductive mechanisms, paving the way for innovations in fertility treatments and improving reproductive efficiency in livestock.












