Analytical Data
-
Gene name
PNPLA3
- Application
-
Alternative Names
PNPLA3；ADPN；C22orf20；1-acylglycerol-3-phosphate O-acyltransferase PNPLA3
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9NST1
-
Expression Region
1-481aa
-
AA Sequence
MYDAERGWSLSFAGCGFLGFYHVGATRCLSEHAPHLLRDARMLFGASAGA LHCVGVLSGIPLEQTLQVLSDLVRKARSRNIGIFHPSFNLSKFLRQGLGK CLPANVHQLISGKIGISLTRVSDGENVLVSDFRSKDEVVDALVCSCFMPF YSGLIPPSFRGVRYVDGGVSDNVPFIDAKTTITVSPFYGEYDICPKVKST NFLHVDITKLSLRLCTGNLYLLSRAFVPPDLKVLGEICLRGYLDAFRFLE EKGICNRPQPGLKSSSEGMDPEVAMPSWANMSLDSSPESAALAVRLEGDE LLDHLRLSILPWDESILDTLSPRLATALSEEMKDKGGYMSKICNLLPIRI MSYVMLPCTLPVESAIAIVQRLVTWLPDMPDDVLWLQWVTSQVFTRVLMC LLPASRSQMPVSSQQASPCTPEQDWPCWTPCSPEGCPAETKAEATPRSIL RSSLNFFLGNKVPAGAEGLSTFPSFSLEKSL
-
Molecular Weight
79 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of PNPLA3 (patatin-like phospholipase domain-containing protein 3) recombinant protein has gained significant attention due to its critical role in lipid metabolism and its association with various liver diseases, particularly non-alcoholic fatty liver disease (NAFLD) and hepatocellular carcinoma (HCC). The PNPLA3 gene encodes a protein that is involved in triglyceride hydrolysis and lipid droplet remodeling in hepatocytes. Variants of this gene, especially the I148M polymorphism, have been linked to increased risk of liver fat accumulation and subsequent liver damage. Understanding the functional mechanisms of PNPLA3, especially through the use of recombinant protein technology, allows researchers to elucidate how these genetic variations influence enzyme activity and lipid processing. This knowledge is crucial for identifying potential therapeutic targets and strategies for liver disease management. Additionally, the production and characterization of PNPLA3 recombinant protein provide insights into protein structure-function relationships and facilitate the exploration of its role in metabolic pathways. Current research efforts are focused on optimizing the expression and purification of this protein, as well as studying its interactions with other cellular components, making it a pivotal focus in the field of metabolic research.
