Analytical Data
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基因名
UEV3
- Application
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别名
Ubiquitin-conjugating enzyme E2 variant 3. UEV-3. EV and lactate/malate dehydrogenase domain-containing Protein
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8IX04
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表达区间
1-357 aa
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氨基酸序列
MEFDCEGLRRLLGKYKFRDLTVEELRNVNVFFPHFKYSMDTYGNTYNIPIRFWILDSHPFAPPICFLKPTANMGILVGKHVDAQGRIYLPYLQNWSHPKSVIVGLIKEMIAKFQEELPMYSLSSSDEARQVDLLAYIAKITEGVSDTNSKSWANHENKTVNKITVVGGGELGIACTLAISAKGIADRLVLLDLSEGTKGATMDLEIFNLPNVEISKDLSASAHSKVVIFTVNSLGSSQSYLDVVQSNVDMFRALVPALGHYSQHSVLLVASQPVEIMTYVTWKLSTFPANRVIGIGCNLDSQRLQYIITNVLKAQTSGKEVWVIGEQGEDKVLTWSGQEEVVSHTSQVQLSNRDIMI
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分子量
66.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research on UEV3 (Ubiquitin E2 Variant 3) recombinant protein has gained significant attention due to its crucial role in cellular processes mediated by ubiquitination, a post-translational modification that regulates protein degradation, cell cycle progression, and DNA repair. UEV3 is a member of the Ubiquitin-conjugating enzyme (E2) family, which is involved in the transfer of ubiquitin molecules to target proteins. Understanding the functions and mechanisms of UEV3 is essential for elucidating its involvement in various diseases, particularly cancer and neurodegenerative disorders, where dysregulation of protein degradation pathways is often observed. Recombinant production of UEV3 allows researchers to obtain this protein in sufficient quantities for biochemical studies and functional assays, facilitating the exploration of its structure, binding properties, and interaction with other cellular components. Additionally, insights gained through UEV3 research could lead to the development of novel therapeutic strategies aimed at modulating ubiquitination processes, thereby offering potential avenues for intervening in disease mechanisms linked to protein misregulation. Overall, the investigation of UEV3 recombinant protein stands at the intersection of molecular biology, biochemistry, and therapeutic innovation, highlighting its significance in advancing our understanding of cellular function and disease pathology.
