Analytical Data
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Gene name
PRDM14
- Application
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Alternative Names
PRDM14；PR domain zinc finger Protein 14
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9GZV8
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Expression Region
2-412aa
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AA Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQ^GPLGSALPRPSEAVPQDKVCYPP ESSPQNLAAYYTPFPSYGHYRNSLATVEEDFQPFRQLEAAASAAPAMPPF PFRMAPPLLSPGLGLQREPLYDLPWYSKLPPWYPIPHVPREVPPFLSSSH EYAGASSEDLGHQIIGGDNESGPCCGPDTLIPPPPADASLLPEGLRTSQL LPCSPSKQSEDGPKPSNQEGKSPARFQFTEEDLHFVLYGVTPSLEHPASL HHAISGLLVPPDSSGSDSLPQTLDKDSLQLPEGLCLMQTVFGEVPHFGVF CSSFIAKGVRFGPFQGKVVNASEVKTYGDNSVMWEIFEDGHLSHFIDGKG GTGNWMSYVNCARFPKEQNLVAVQCQGHIFYESCKEIHQNQELLVWYGDC YEKFLDIPVSLQVTEPGKQPSGPSEESAEGYRCERCGKVFTYK
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Molecular Weight
72 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PRDM14 is a zinc-finger protein that plays a pivotal role in regulating gene expression during embryonic development and pluripotency in stem cells. It is a member of the PRDM (PR domain containing) family, which is involved in various biological processes including cell differentiation, proliferation, and reprogramming. The interest in PRDM14 has surged due to its critical function in maintaining the properties of embryonic stem cells and its involvement in germ cell development. Abnormal expression of PRDM14 has been associated with several types of cancer, making it a potential biomarker and therapeutic target. Recent research has focused on the recombinant production of PRDM14 to better understand its structure-function relationship and to elucidate its role in cellular mechanisms. The reconstitution of PRDM14 as a recombinant protein allows for detailed biochemical characterization, providing insights into its interaction with other molecular partners and its regulatory pathways. This research not only enhances our understanding of PRDM14’s biological functions but also paves the way for potential applications in regenerative medicine and cancer therapy. Studies are exploring the use of PRDM14 in enhancing the reprogramming efficiency of somatic cells into induced pluripotent stem cells (iPSCs), highlighting its significance in stem cell biology and therapeutic developments. Hence, the investigation of PRDM14 and its recombinant protein forms serves as a crucial step in uncovering novel strategies for manipulating cell fate and advancing stem cell research.
