Analytical Data
-
Gene name
UBE3B
- Application
-
Alternative Names
UBE3B; Ubiquitin-Protein ligase E3B; EC 2.3.2.26; HECT-type ubiquitin transferase E3B
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q7Z3V4
-
Expression Region
1-244 aa
-
AA Sequence
MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQCCDGLFPDLVSYAPHNNPVRWSVGRSWYDWQLSR
-
Molecular Weight
53.3 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
UBE3B, a member of the E3 ubiquitin ligase family, plays a critical role in the ubiquitin-proteasome system, which is essential for regulating protein degradation, cellular processes, and maintaining homeostasis. Dysregulation of E3 ligases like UBE3B has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting their potential as therapeutic targets. Recent studies have demonstrated that UBE3B is involved in modulating cellular responses to stress and has a role in regulating gene expression and apoptosis. Given its significance in these pathways, researchers are increasingly focused on characterizing UBE3B as a recombinant protein to further investigate its structural and functional properties. The production of UBE3B as a recombinant protein allows for in-depth analysis of its enzymatic activity, substrate specificity, and interaction with other cellular components. Understanding the molecular mechanisms underpinning UBE3B’s function is crucial for developing targeted therapies that could mitigate its associated pathologies. Thus, the study of UBE3B is not only pivotal for basic science but also holds promise for advancing therapeutic strategies in the treatment of diseases related to aberrant protein degradation pathways.
