Analytical Data
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Gene name
U2AF2
- Application
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Alternative Names
(U2 auxiliary factor 65 kDa subunit)(hU2AF(65)(hU2AF65)(U2 snRNP auxiliary factor large subunit)
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P26368
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Expression Region
2-475 aa
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AA Sequence
SDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSASRDRRRRSKPLTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW
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Molecular Weight
53.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
U2AF2, also known as U2 auxiliary factor 2, is a crucial splicing factor involved in the regulation of pre-mRNA splicing. It functions as a vital component of the spliceosome, interacting with the U2 snRNP (small nuclear ribonucleoprotein) to facilitate the recognition of the branch point and upstream 3' splice site during the splicing process. Recent research has highlighted the role of U2AF2 in various biological processes, including gene expression regulation and the modulation of alternative splicing events, which have significant implications in cellular differentiation, development, and response to stress. Alterations or mutations in U2AF2 have been implicated in several diseases, including cancer, where aberrant splicing can lead to the production of oncogenic isoforms and contribute to tumor progression. Given its importance, the study of U2AF2 and the characterization of its recombinant protein are critical for understanding its functional mechanisms, interactions with other splicing factors, and its potential as a therapeutic target. Identifying and characterizing U2AF2's structural and functional properties through recombinant protein studies can provide insights into how it influences splicing decisions and pathways, with significant implications for developing novel interventions in splicing-related diseases. Understanding U2AF2's role at the molecular level is a promising area of research that could lead to advancements in targeted therapies for diseases associated with splicing dysregulation.
