Analytical Data
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Gene name
TUBA3D
- Application
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Alternative Names
Tubulin alpha-3D chain. EC:3.6.5.-. Alpha-tubulin 3D. Cleaved into: Detyrosinated tubulin alpha-3D chain
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0DPH8
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Expression Region
1-450 aa
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AA Sequence
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKYDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGVDSVEAEAEEGEEY
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Molecular Weight
76.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TUBA3D, a member of the tubulin protein family, plays a crucial role in the formation of microtubules, which are essential components of the cytoskeleton in eukaryotic cells. Research on TUBA3D focuses on its unique structural and functional properties, particularly in relation to its involvement in cell division, intracellular transport, and the maintenance of cell shape. The study of TUBA3D recombinant proteins allows scientists to explore its molecular mechanisms and interactions with other proteins, which can provide insights into various cellular processes and disorders. Given that aberrations in tubulin dynamics are associated with numerous diseases, including cancer and neurodegenerative disorders, TUBA3D serves as a potential target for therapeutic interventions. Additionally, understanding the functional diversity of TUBA3D can aid in elucidating the evolutionary adaptations of tubulin proteins across different species, highlighting the importance of this research in both basic biology and applied sciences. Through advanced techniques such as X-ray crystallography and cryo-electron microscopy, the structural characterization of TUBA3D and its complexes can pave the way for the development of novel drugs that modulate microtubule dynamics, offering promising avenues for clinical applications. As the scientific community continues to unravel the complexities of microtubule biology, the study of TUBA3D remains a significant focus, contributing to our understanding of cellular architecture and its implications for health and disease.
