Analytical Data
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Gene name
GLYT1
- Application
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Alternative Names
GLYT1；Sodium- and chloride-dependent glycine transporter 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P26572
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Expression Region
1-445aa
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AA Sequence
MLKKQSAGLVLWGAILFVAWNALLLLFFWTRPAPGRPPSVSALDGDPASLTREVIRLAQDAEVELERQRGLLQQIGDALSSQRGRVPTAAPPAQPRVPVTPAPAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGHEETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFRFPAAVVVEDDLEVAPDFFEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLYRTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPLTWEGYDPSWN
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Molecular Weight
50.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GLYT1, or Glycine Transporter 1, is a crucial membrane protein that plays a significant role in the regulation of glycine levels in the central nervous system (CNS). Glycine acts as an inhibitory neurotransmitter and is involved in various physiological functions, including motor control, sensory processing, and modulation of NMDA receptor activity. Given the importance of glycine in neurotransmission and its implications in neuropsychiatric disorders, understanding GLYT1's structure and function is critical. The recombinant production of GLYT1 protein facilitates in-depth studies into its transport mechanisms, substrate specificity, and interactions with pharmacological agents. These studies can provide insights into the transporter's role in pathologies such as schizophrenia, epilepsy, and other CNS disorders. Furthermore, GLYT1 has emerged as a potential therapeutic target, as modulating its activity could restore glycine levels and improve synaptic transmission. Therefore, investigating the biochemical properties of GLYT1 through recombinant techniques is essential for advancing therapeutic strategies and developing novel drugs that target this transporter, ultimately paving the way for innovative approaches to treat various neurological conditions.
