Analytical Data
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基因名
TSEN54
- Application
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别名
HsSen54; PCH2A; PCH4; sen54; SEN54 homolog; SEN54_HUMAN; SEN54L; tRNA-intron endonuclease SEN54; tRNA-splicing endonuclease subunit SEN54; Tsen54; TSEN54 tRNA splicing endonuclease subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q7Z6J9
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表达区间
1-526 aa
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氨基酸序列
MEPEPEPAAV EVPAGRVLSA RELFAARSRS QKLPQRSHGP KDFLPDGSAA QAERLRRCRE ELWQLLAEQR VERLGSLVAA EWRPEEGFVE LKSPAGKFWQ TMGFSEQGRQ RLHPEEALYL LECGSIHLFH QDLPLSIQEA YQLLLTDHTV TFLQYQVFSH LKRLGYVVRR FQPSSVLSPY ERQLNLDASV QHLEDGDGKR KRSSSSPRSI NKKAKALDNS LQPKSLAASS PPPCSQPSQC PEEKPQESSP MKGPGGPFQL LGSLGPSPGP AREGVGCSWE SGRAENGVTG AGKRRWNFEQ ISFPNMASDS RHTLLRAPAP ELLPANVAGR ETDAESWCQK LNQRKEKLSR REREHHAEAA QFQEDVNADP EVQRCSSWRE YKELLQRRQV QRSQRRAPHL WGQPVTPLLS PGQASSPAVV LQHISVLQTT HLPDGGARLL EKSGGLEIIF DVYQADAVAT FRKNNPGKPY ARMCISGFDE PVPDLCSLKR LSYQSGDVPL IFALVDHGDI SFYSFRDFTL PQDVGH
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分子量
58.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The TSEN54 protein is a crucial component of the tRNA splicing endonuclease complex, which plays a significant role in the maturation of tRNA molecules by catalyzing the removal of introns from precursor tRNA transcripts. Mutations in the TSEN54 gene have been linked to a rare neurodegenerative disorder known as pontocerebellar hypoplasia (PCH), characterized by underdevelopment of specific brain regions, resulting in severe developmental delays and motor impairments. Understanding the structure and function of the TSEN54 protein is therefore essential for elucidating the molecular mechanisms underlying PCH and related disorders. Recent studies have employed recombinant protein techniques to produce TSEN54, allowing researchers to investigate its enzymatic activity, interaction with other components of the tRNA splicing pathway, and the impact of disease-associated mutations on its function. This research is pivotal not only for understanding the basic biology of tRNA processing but also for identifying potential therapeutic avenues for conditions stemming from TSEN54-related dysfunction. Given the critical role of tRNA maturation in cellular health and the implications of its dysregulation in disease, the comprehensive study of TSEN54 is a vital focus area in both molecular biology and medical research, contributing to our broader understanding of genetic disorders and potential interventions.
