Analytical Data
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Gene name
TSEN34
- Application
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Alternative Names
HsSen34; LENG 5; LENG5; Leukocyte receptor cluster (LRC) member 5; Leukocyte receptor cluster member 5; SEN 34; SEN34; SEN34_HUMAN; SEN34L; tRNA intron endonuclease Sen34; tRNA splicing endonuclease 34 homolog; tRNA splicing endonuclease subunit 34; tRNA splicing endonuclease subunit Sen34; tRNA-intron endonuclease Sen34; tRNA-splicing endonuclease subunit Sen34; TSEN 34; Tsen34
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BSV6
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Expression Region
1-310 aa
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AA Sequence
MLVVEVANGR SLVWGAEAVQ ALRERLGVGG RTVGALPRGP RQNSRLGLPL LLMPEEARLL AEIGAVTLVS APRPDSRHHS LALTSFKRQQ EESFQEQSAL AAEARETRRQ ELLEKITEGQ AAKKQKLEQA SGASSSQEAG SSQAAKEDET SDGQASGEQE EAGPSSSQAG PSNGVAPLPR SALLVQLATA RPRPVKARPL DWRVQSKDWP HAGRPAHELR YSIYRDLWER GFFLSAAGKF GGDFLVYPGD PLRFHAHYIA QCWAPEDTIP LQDLVAAGRL GTSVRKTLLL CSPQPDGKVV YTSLQWASLQ
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Molecular Weight
33.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TSEN34, or tRNA splicing endonuclease 34, is a crucial enzyme involved in the maturation of tRNA by facilitating the removal of introns from precursor tRNA molecules. The significance of TSEN34 has garnered attention due to its role in tRNA processing, which is essential for proper protein synthesis in all cells. Mutations or dysfunctions in the TSEN34 gene are linked to various diseases, particularly neurodegenerative disorders such as pontocerebellar hypoplasia, which highlight the enzyme's importance in maintaining cellular homeostasis. Studies have shown that TSEN34 operates as part of a multi-subunit complex that acts on specific tRNA substrates, ensuring their correct maturation and function. The understanding of TSEN34's structure, function, and the mechanisms by which it interacts with other cell components can reveal potential therapeutic targets for diseases associated with tRNA misprocessing. Research into TSEN34 and its biochemical pathways is not only critical for elucidating fundamental cellular processes but also has implications for developing interventions for tRNA-related pathologies. As a result, TSEN34 continues to be a focal point in molecular biology research, with ongoing studies aiming to unravel its precise mechanisms and potential roles in both health and disease.
