Analytical Data
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Gene name
TSEN2
- Application
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Alternative Names
HsSen2; MGC2776; MGC4440; SEN2; SEN2_HUMAN; SEN2L ; tRNA intron endonuclease Sen2; tRNA splicing endonuclease 2 homolog (SEN2; S. cerevisiae); tRNA splicing endonuclease subunit Sen2; tRNA-intron endonuclease sen2; tRNA-splicing endonuclease subunit Sen2; TSEN 2; TSEN2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NCE0
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Expression Region
1-465 aa
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AA Sequence
MAEAVFHAPK RKRRVYETYE SPLPIPFGQD HGPLKEFKIF RAEMINNNVI VRNAEDIEQL YGKGYFGKGI LSRSRPSFTI SDPKLVAKWK DMKTNMPIIT SKRYQHSVEW AAELMRRQGQ DESTVRRILK DYTKPLEHPP VKRNEEAQVH DKLNSGMVSN MEGTAGGERP SVVNGDSGKS GGVGDPREPL GCLQEGSGCH PTTESFEKSV REDASPLPHV CCCKQDALIL QRGLHHEDGS QHIGLLHPGD RGPDHEYVLV EEAECAMSER EAAPNEELVQ RNRLICRRNP YRIFEYLQLS LEEAFFLVYA LGCLSIYYEK EPLTIVKLWK AFTVVQPTFR TTYMAYHYFR SKGWVPKVGL KYGTDLLLYR KGPPFYHASY SVIIELVDDH FEGSLRRPLS WKSLAALSRV SVNVSKELML CYLIKPSTMT DKEMESPECM KRIKVQEVIL SRWVSSRERS DQDDL
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Molecular Weight
53.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TSEN2 (tRNA splicing endonuclease 2) is an essential protein involved in the splicing of precursor tRNA, a critical process for proper protein synthesis in eukaryotic cells. Mutations in the TSEN2 gene have been linked to various neuromuscular disorders, particularly in the context of pontocerebellar hypoplasia, a group of rare neurodegenerative conditions. Given its role in tRNA processing, TSEN2 is vital for maintaining cellular function and overall cellular health; disruptions can lead to impaired translation and energy metabolism. Research on TSEN2-recombinant proteins aims to elucidate its structural and functional characteristics, providing insights into the mechanisms underlying its role in tRNA splicing. Understanding the biochemical properties of the recombinant TSEN2 protein can shed light on the pathogenic mechanisms associated with TSEN2 mutations. Furthermore, this research may contribute to the development of therapeutic strategies targeting the molecular pathways involved in tRNA splicing and related disorders. Investigating TSEN2 in this manner not only enhances our knowledge of fundamental cellular processes but also aids in the identification of potential biomarkers and therapeutic targets for diseases linked to TSEN2 dysfunction.
