Analytical Data
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基因名
TSEN2
- Application
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别名
HsSen2; MGC2776; MGC4440; SEN2; SEN2_HUMAN; SEN2L ; tRNA intron endonuclease Sen2; tRNA splicing endonuclease 2 homolog (SEN2; S. cerevisiae); tRNA splicing endonuclease subunit Sen2; tRNA-intron endonuclease sen2; tRNA-splicing endonuclease subunit Sen2; TSEN 2; TSEN2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8NCE0
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表达区间
1-465 aa
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氨基酸序列
MAEAVFHAPK RKRRVYETYE SPLPIPFGQD HGPLKEFKIF RAEMINNNVI VRNAEDIEQL YGKGYFGKGI LSRSRPSFTI SDPKLVAKWK DMKTNMPIIT SKRYQHSVEW AAELMRRQGQ DESTVRRILK DYTKPLEHPP VKRNEEAQVH DKLNSGMVSN MEGTAGGERP SVVNGDSGKS GGVGDPREPL GCLQEGSGCH PTTESFEKSV REDASPLPHV CCCKQDALIL QRGLHHEDGS QHIGLLHPGD RGPDHEYVLV EEAECAMSER EAAPNEELVQ RNRLICRRNP YRIFEYLQLS LEEAFFLVYA LGCLSIYYEK EPLTIVKLWK AFTVVQPTFR TTYMAYHYFR SKGWVPKVGL KYGTDLLLYR KGPPFYHASY SVIIELVDDH FEGSLRRPLS WKSLAALSRV SVNVSKELML CYLIKPSTMT DKEMESPECM KRIKVQEVIL SRWVSSRERS DQDDL
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分子量
53.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TSEN2 (tRNA splicing endonuclease 2) is an essential protein involved in the splicing of precursor tRNA, a critical process for proper protein synthesis in eukaryotic cells. Mutations in the TSEN2 gene have been linked to various neuromuscular disorders, particularly in the context of pontocerebellar hypoplasia, a group of rare neurodegenerative conditions. Given its role in tRNA processing, TSEN2 is vital for maintaining cellular function and overall cellular health; disruptions can lead to impaired translation and energy metabolism. Research on TSEN2-recombinant proteins aims to elucidate its structural and functional characteristics, providing insights into the mechanisms underlying its role in tRNA splicing. Understanding the biochemical properties of the recombinant TSEN2 protein can shed light on the pathogenic mechanisms associated with TSEN2 mutations. Furthermore, this research may contribute to the development of therapeutic strategies targeting the molecular pathways involved in tRNA splicing and related disorders. Investigating TSEN2 in this manner not only enhances our knowledge of fundamental cellular processes but also aids in the identification of potential biomarkers and therapeutic targets for diseases linked to TSEN2 dysfunction.
