Analytical Data
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基因名
TRMT1
- Application
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别名
2)G26)dimethyltransferase; 2-dimethylguanosine-26 methyltransferase; FLJ20244; N(2) N(2) dimethylguanosine tRNA methyltransferase; N(2)-N(2)) methyltransferase; TRM 1; TRM1 tRNA methyltransferase 1 homolog; TRM1_HUMAN; TRMT 1; Trmt1; tRNA (guanine(26)-N(2))-dimethyltransferase; tRNA 2 2 dimethylguanosine 26 methyltransferase; tRNA 2; tRNA(guanine 26 N(2) N(2)) methyltransferase; tRNA(guanine-26; tRNA(m(2 2)G26)dimethyltransferase; tRNA(m(2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NXH9
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表达区间
1-659 aa
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氨基酸序列
MQGSSLWLSL TFRSARVLSR ARFFEWQSPG LPNTAAMENG TGPYGEERPR EVQETTVTEG AAKIAFPSAN EVFYNPVQEF NRDLTCAVIT EFARIQLGAK GIQIKVPGEK DTQKVVVDLS EQEEEKVELK ESENLASGDQ PRTAAVGEIC EEGLHVLEGL AASGLRSIRF ALEVPGLRSV VANDASTRAV DLIRRNVQLN DVAHLVQPSQ ADARMLMYQH QRVSERFDVI DLDPYGSPAT FLDAAVQAVS EGGLLCVTCT DMAVLAGNSG ETCYSKYGAM ALKSRACHEM ALRIVLHSLD LRANCYQRFV VPLLSISADF YVRVFVRVFT GQAKVKASAS KQALVFQCVG CGAFHLQRLG KASGVPSGRA KFSAACGPPV TPECEHCGQR HQLGGPMWAE PIHDLDFVGR VLEAVSANPG RFHTSERIRG VLSVITEELP DVPLYYTLDQ LSSTIHCNTP SLLQLRSALL HADFRVSLSH ACKNAVKTDA PASALWDIMR CWEKECPVKR ERLSETSPAF RILSVEPRLQ ANFTIREDAN PSSRQRGLKR FQANPEANWG PRPRARPGGK AADEAMEERR RLLQNKRKEP PEDVAQRAAR LKTFPCKRFK EGTCQRGDQC CYSHSPPTPR VSADAAPDCP ETSNQTPPGP GAAAGPGID
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分子量
72.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TRMT1 (tRNA methyltransferase 1) is an essential enzyme that plays a crucial role in tRNA maturation by catalyzing the methylation of specific adenine residues. This post-transcriptional modification is vital for maintaining the stability and efficiency of tRNA molecules, ultimately influencing protein synthesis and cellular function. Mutations in the TRMT1 gene have been linked to various human diseases, including neurological disorders and developmental abnormalities, highlighting the importance of understanding this enzyme's structure and function. Recent studies have focused on the recombinant expression and characterization of TRMT1, as obtaining sufficient amounts of pure protein is critical for in-depth biochemical and structural analyses. Through recombinant technology, researchers aim to elucidate the enzyme's active site, substrate specificity, and overall catalytic mechanism. This knowledge not only enhances our understanding of tRNA modification pathways but also paves the way for potential therapeutic approaches targeting TRMT1-related diseases. The ongoing exploration of TRMT1's role in cellular processes underscores the significance of this enzyme in both basic biology and clinical research, emphasizing the need for continued investigation into its properties and applications.
