Analytical Data
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Gene name
ZNF254
- Application
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Alternative Names
ZNF254; BMZF5; ZNF539; ZNF91L; Zinc finger Protein 254; Bone marrow zinc finger 5; BMZF-5; Hematopoietic cell-derived zinc finger Protein 1; HD-ZNF1; Zinc finger Protein 539; Zinc finger Protein 91-like
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75437
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Expression Region
1-659 aa
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AA Sequence
MPGPPRSLEM GLLTFRDVAI EFSLEEWQHL DIAQQNLYRN VMLENYRNLA FLGIAVSKPD LITCLEQGKE PWNMKRHEMV DEPPGMCPHF AQDLWPEQGM EDSFQKAILR RYGKYGHENL QLRKGCKSVD EYKVNKEGYN GLNQCFTTAQ SKVFQCDKYL KVFYKFLNSN RPKIRHTEKK SFKCKKRVKL FCMLSHKTQH KSIYHREKSY KCKECGKTFN WSSTLTNHRK IYTEEKPYKC EEYNKSPKQL STLTTHEIIH AGEKLYKCEE CGEAFNRSSN LTTHKIIHTG EKPYKCEECG KAFIWSSTLT EHKKIHTRKK PYKCEECGKA FIWSSTLTRH KRMHTGEKPY KCEECGKAFS QSSTLTTHKI IHTGEKRYKC LECGKAFKQL STLTTHKIIH VGEKLYKCEE CGKGFNRSSN LTTHKIIHTG EKPYKCEECG KAFIWSSTLT KHKRIHTREK PYKCEECGKA FIWSSTLTRH KRMHTGEKPY KCEECGKSFS QSSTLTTHKI IHTGEKPYKC EECGKAFNWS STLTKHKIIH TEEKPYKCEK CGKAFKQSSI LTNHKRIHTG EKPYKCEECG KSFNRSSTFT KHKVIHTGVK PYKCEECGKA FFWSSTLTKH KRIHTGEQPY KWEKFGKAFN RSSHLTTDKI THWREILQV
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Molecular Weight
77.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ZNF254, a member of the zinc finger protein family, has garnered significant interest in recent years due to its potential role in gene regulation and epigenetic modulation. Research indicates that ZNF254 functions as a transcriptional repressor, influencing the expression of genes involved in critical biological processes such as cell differentiation, development, and responses to environmental stimuli. Mutations or dysregulation of ZNF254 have been associated with various diseases, including cancer, highlighting the importance of understanding its functional mechanisms. The recombinant expression of ZNF254 in model systems allows for the elucidation of its structure and function, enabling researchers to explore its interactions with DNA and other cellular components. Furthermore, studying the recombinantly produced ZNF254 protein facilitates the development of potential therapeutic strategies aimed at modulating its activity for clinical applications. This research area is particularly promising as it integrates molecular biology, genetics, and biochemistry, offering insights into the fundamental principles of gene regulation and its implications in health and disease.
