Analytical Data
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Gene name
ZNF253
- Application
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Alternative Names
ZNF253; BMZF1; ZNF411; Zinc finger Protein 253; Bone marrow zinc finger 1; BMZF-1; Zinc finger Protein 411
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75346
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Expression Region
1-499 aa
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AA Sequence
MGPLQFRDVA IEFSLEEWHC LDTAQRNLYR DVMLENYRNL VFLGIVVSKP DLVTCLEQGK KPLTMERHEM IAKPPVMSSH FAQDLWPENI QNSFQIGMLR RYEECRHDNL QLKKGCKSVG EHKVHKGGYN GLNQCLTTTQ KEIFQCDKYG KVFHKFSNSN TYKTRHTGIN LFKCIICGKA FKRSSTLTTH KKIHTGEKPY RCEECGKAFN QSANLTTHKR IHTGEKPYRC EECGKAFKQS SNLTTHKKIH TGEKPYKCEE CGKAFNRSTD LTTHKIVHTG EKPYKCEECG KAFKHPSHVT THKKIHTRGK PYNCEECGKS FKHCSNLTIH KRIHTGEKPY KCEECGKAFH LSSHLTTHKI LHTGEKPYRC RECGKAFNHS TTLFSHEKIH TGEKPYKCDE CGKTFTWPSI LSKHKRTHTG EKPYKCEECG KSFTASSTLT THKRIHTGEK PYKCEECGKA FNWSSDLNKH KKIHIERKPY IVKNVTDLLN VPPLLISIR
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Molecular Weight
57.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ZNF253, a member of the zinc finger protein family, plays a crucial role in gene regulation and is implicated in various biological processes, including cell differentiation and development. Its unique structure, characterized by multiple zinc finger motifs, allows it to interact with DNA and regulate target gene expression. Research into ZNF253 has gained traction due to its potential involvement in oncogenesis and its association with certain diseases, such as cancer. Understanding the function and mechanisms of ZNF253 can provide insights into its role in cellular processes and its potential as a therapeutic target. The recombinant expression of ZNF253 in various systems has facilitated the study of its structure-function relationships, protein interactions, and regulatory pathways. Moreover, characterizing the protein allows for deeper exploration into its interactions with other cellular components, which can illuminate its role in gene expression modulation. Recent advancements in protein engineering and purification techniques have enabled researchers to produce large quantities of functional ZNF253, paving the way for detailed biochemical analyses. Investigations using ZNF253 recombinant protein have the potential to reveal novel insights into its transcriptional regulatory functions and its implications in health and disease, highlighting the importance of this protein in both basic and applied biomedical research.
