Analytical Data
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基因名
DGKe
- Application
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别名
DGKe；DAGK5；Diacylglycerol kinase epsilon
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P52429
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表达区间
1-567aa
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氨基酸序列
MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVFITFWCSLQRSRRQLH RRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLR KADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPK LCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYLTSINQMRK DKKTDYEVLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVF DVTKTPPIKALQLCTLLPYYSARVLVCGGDGTVGWVLDAVDDMKIKGQEK YIPQVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRW KVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSR ILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVALPSLEGIIVLNIG YWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFR IGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHAMMLYFSGEQT DDDISSTSDQEDIKATE
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DGKe (diacylglycerol kinase epsilon) is an important enzyme implicated in various cellular signaling pathways by regulating diacylglycerol (DAG) levels, a key lipid second messenger. Research on DGKe has gained traction due to its potential roles in several physiological processes, including T cell activation, immune response, and neuron signaling. Genetic studies have associated DGKe with diseases such as cancer and autoimmune disorders, highlighting its significance in pathophysiology. The enzyme's unique features, such as its ability to regulate DAG metabolism in specific cellular contexts, make it a compelling target for therapeutic interventions. Recombining DGKe for experimental purposes allows researchers to probe its functional mechanisms, explore its interactions with other proteins, and devise potential strategies to manipulate its activity. Understanding DGKe's structure and function could pave the way for the development of novel treatments for related diseases, driving ongoing research into its biochemical properties and regulatory roles in health and disease.
