Analytical Data
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基因名
UBE1L
- Application
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别名
UBE1L；UBE1L；UBE2；Ubiquitin-like modifier-activating enzyme 7
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P41226
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表达区间
1-1012aa
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氨基酸序列
MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKN LVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNR AVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAAD TRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANT HYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRG GAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKF QHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVA LSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGE LLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKV FALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAA RGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAA RCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCT VRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLT LLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLED GTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTAL RELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVG PPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQII PAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFA PAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGS ALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDD EDTAFPPLHYEL
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分子量
138 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UBE1L, or Ubiquitin-Activating Enzyme 1 L, is a critical enzyme involved in the ubiquitin-proteasome pathway, which regulates protein degradation and various cellular processes such as cell cycle progression, DNA repair, and responses to stress. The study of UBE1L has gained traction due to its significant role in maintaining cellular homeostasis and its implications in several diseases, including cancer and neurodegenerative disorders. Recent research has highlighted the potential of UBE1L as a therapeutic target, as its dysregulation can lead to abnormal protein accumulation and subsequent pathologies. The recombinant production of UBE1L allows for a detailed study of its enzymatic activity, substrate specificity, and mechanisms of action. This research not only aids in understanding the fundamental biology of the ubiquitin system but also opens avenues for the development of novel therapeutic strategies aimed at modulating UBE1L activity. Additionally, recombinant UBE1L can serve as a valuable tool for high-throughput screening assays to identify small molecule inhibitors or activators, further enhancing our understanding of its biological functions and therapeutic potential.
